自制多孔微球高密度培养Vero细胞的初步研究

多孔微球是动物细胞高密度培养的有效手段,它是1985年由Verax公司开创的,最初用于流化床生物反应器生产单克隆抗体,后来又出现了Percell和Siran系统系列多孔微球,并且使用的反应器种类和生产的产品都在增加,于是便成为一种新型的细胞培养手段而日益受到人们的重视。为此,我们在利用微载体进行Vero细胞高密度培养的同时,又对多孔微球的制备和培养工艺作了初步探索。

Preliminary Study of High Density Cultivation of Vero Cells with Self-made Porous Microspheres

During studying high density cultivation of Vero cells with Biosilon micro-carrier we also developed a kind of gelatin porous microsphere. Their diameter range were 76-150um (18. 6%) and 150-200um (45. 6%) respectively. Stained with MTT, it showed that Vero cells could proliferated both on the exterior surface and in the interior surface of the porous microspheres. When the concentration of porous microsphere was 5 mg/ml, inoculum cell density was 1.23x106/ml, Vero cell density could reach 1. 24x107/ml, after 12 days of cultivation in a modified Wheaton spinner bottle. The dead cell density was about 1 x 105/ml, about 0. 8% of the total cells. These experiments proved that the porous microsphere made by our laboratory was also suitable for high density cultivation of Vero cells.