| 海绵内/共生稀有放线菌Dermacoccus sp. X4次级代谢产物分离纯化及结构解析 |
| |
| 引用本文： | 张艳凤,许勇,陈雷,胡俊,张学成,房伟,方泽民,肖亚中.海绵内/共生稀有放线菌Dermacoccus sp. X4次级代谢产物分离纯化及结构解析[J].生物工程学报,2016,32(5):599-609. |
| |
| 作者姓名： | 张艳凤 许勇 陈雷 胡俊 张学成 房伟 方泽民 肖亚中 |
| |
| 作者单位： | 1 安徽大学 生命科学学院，安徽 合肥 230601；2 安徽省微生物与生物催化工程技术研究中心，安徽 合肥 230039,1 安徽大学 生命科学学院，安徽 合肥 230601；2 安徽省微生物与生物催化工程技术研究中心，安徽 合肥 230039,1 安徽大学 生命科学学院，安徽 合肥 230601；2 安徽省微生物与生物催化工程技术研究中心，安徽 合肥 230039,1 安徽大学 生命科学学院，安徽 合肥 230601；2 安徽省微生物与生物催化工程技术研究中心，安徽 合肥 230039,1 安徽大学 生命科学学院，安徽 合肥 230601；2 安徽省微生物与生物催化工程技术研究中心，安徽 合肥 230039,1 安徽大学 生命科学学院，安徽 合肥 230601；2 安徽省微生物与生物催化工程技术研究中心，安徽 合肥 230039,1 安徽大学 生命科学学院，安徽 合肥 230601；2 安徽省微生物与生物催化工程技术研究中心，安徽 合肥 230039,1 安徽大学 生命科学学院，安徽 合肥 230601；2 安徽省微生物与生物催化工程技术研究中心，安徽 合肥 230039 |
| |
| 基金项目： | 国家高技术研究发展计划 (863计划) (No. 2013AA092901)，教育部留学回国人员科研项目，安徽大学人才引进计划 (No. 32030066)，安徽大学211工程创新团队计划项目经费资助。 |
| |
| 摘 要： | 从中国南海西沙群岛10 m深海水中采集得到一株未鉴定海绵样品,对其进行共附生微生物的分离,得到真菌及细菌共16株。通过分子生物学鉴定及菌株发酵液抑菌活性测定,发现其中一株稀有放线菌皮生球属菌株Dermacoccus sp.X4对金黄色葡萄球菌具有较好抑菌活性。大量发酵制备该菌株发酵液,通过硅胶分配层析、ODS反相层析、SephadexTMLH-20凝胶过滤层析及C18反相层析等分离方法对发酵液成分进行分离,并使用液相质谱连用、一维核磁及二维核磁分析对分离得到的单一化合物进行鉴定,确定二酮哌嗪类化合物1个,吲哚酸酯类化合物2个。本研究中吲哚酸酯类化合物为首次从微生物次级代谢产物中得到,为丰富海洋微生物药用资源作出贡献。
|
| 关 键 词： | 海绵共附生微生物，次级代谢产物，抑菌活性，分离纯化，结构解析 |
| 收稿时间： | 2015/9/10 0:00:00 |
Isolation, identification and structural characterization of secondary metabolites from amarine sponge-derived rare actinobacterium Dermacoccus sp. X4 |
| |
| Yanfeng Zhang,Yong Xu,Lei Chen,Jun Hu,Xuecheng Zhang,Wei Fang,Zemin Fang and Yazhong Xiao.Isolation, identification and structural characterization of secondary metabolites from amarine sponge-derived rare actinobacterium Dermacoccus sp. X4[J].Chinese Journal of Biotechnology,2016,32(5):599-609. |
| |
| Authors: | Yanfeng Zhang Yong Xu Lei Chen Jun Hu Xuecheng Zhang Wei Fang Zemin Fang and Yazhong Xiao |
| |
| Institution: | 1 School of Life Science, Anhui University, Hefei 230601, Anhui, China; 2 Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei 230039, Anhui, China,1 School of Life Science, Anhui University, Hefei 230601, Anhui, China; 2 Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei 230039, Anhui, China,1 School of Life Science, Anhui University, Hefei 230601, Anhui, China; 2 Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei 230039, Anhui, China,1 School of Life Science, Anhui University, Hefei 230601, Anhui, China; 2 Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei 230039, Anhui, China,1 School of Life Science, Anhui University, Hefei 230601, Anhui, China; 2 Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei 230039, Anhui, China,1 School of Life Science, Anhui University, Hefei 230601, Anhui, China; 2 Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei 230039, Anhui, China,1 School of Life Science, Anhui University, Hefei 230601, Anhui, China; 2 Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei 230039, Anhui, China and 1 School of Life Science, Anhui University, Hefei 230601, Anhui, China; 2 Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei 230039, Anhui, China |
| |
| Abstract: | We isolated and identified the symbiotic and adnascent microorganisms from an unidentified sponge collected from 10-meter-deep seawater of the Paracel Islands in China. A total of 16 strains were obtained and identified. Through bacteriostatic activity assay, one of the strains, Dermacoccus sp. X4, was found to effectively inhibit the growth of Staphylococcus aureus. Subsequently, its secondary metabolites were purified by silica gel partition, octadecylsilane (ODS) reverse phase, SephadexTMLH-20 size exclusion, and C18 reverse phase chromatography. Using liquid chromatography, mass spectrometry, and nuclear magnetic resonance, three of the purified compounds were structurally characterized to be one 3-(4-hydroxybenzyl) hexahydropyrrolo 1,2-a]pyrazine-1,4-dione and two indole acid glycerides. This is the first report about indole acid glyceride isolated from microbial secondary metabolites, enriching marine drug candidate resources. |
| |
| Keywords: | sponge associated microorganism secondary metabolite bacteriostatic activity isolation and purification structural characterization |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《生物工程学报》浏览原始摘要信息 |
| 点击此处可从《生物工程学报》下载免费的PDF全文 |
|
