|
|||||
|
|
| 狂犬病病毒抗体胶体金检测试纸的制备 | |
| 引用本文： | 王铁成,张涛,杨松涛,王化磊,高玉伟,孙玮,靳晓霞,赵平森,冯娜,黄耕,邹啸环,夏咸柱.狂犬病病毒抗体胶体金检测试纸的制备[J].生物工程学报,2011,27(5):799-804. |
| 作者姓名： | 王铁成 张涛 杨松涛 王化磊 高玉伟 孙玮 靳晓霞 赵平森 冯娜 黄耕 邹啸环 夏咸柱 |
| 作者单位： | 1. 中国人民解放军军事医学科学院军事兽医研究所,吉林省人兽共患病预防与控制重点实验室,长春,130122 2. 中国医学科学院,北京协和医院实验动物研究所,北京,100021 3. 吉林大学,畜牧兽医学院,长春,130062 |
| 基金项目： | 国家科技部支撑计划项目 (No. 2006BAD6A09)，农业公益性行业项目 (No. 200803014) 资助。 |
| 摘 要： | 通过胶体金免疫层析技术建立一种特异、便捷、快速的狂犬病病毒抗体检测方法，对犬等动物免疫狂犬病疫苗后的抗体水平监测提供参考。用醋酸锌沉淀法沉淀狂犬病病毒CVS11，Sepharose 4FF进行层析纯化。用柠檬酸三钠还原法制备的胶体金，标记纯化的狂犬病病毒，喷于试纸的结合释放垫 (金标垫)，将SPA (葡萄球菌表面A蛋白) 和纯化的兔抗狂犬病病毒IgG分别喷于试纸的T (检测线) 处和C (对照线) 处，组装试纸条。用制备的试纸条对261份犬血清进行检测，与快速荧光灶抑制试验 (RFFIT) 检测的结果一致；对已知效价的犬狂犬病毒中和抗体 (VNA) 大于0.5 IU，结果为阳性；对狂犬病病毒中和抗体 (VNA) 小于0.5 IU/mL的血清，结果为阴性。制备的狂犬病病毒抗体胶体金检测试纸检测犬血清抗体，具有特异、便捷、快速的特点，能够检测出狂犬病病毒中和抗体大于0.5 IU的血清，适用于临床犬血清抗体水平监测，具有良好的应用前景。 |
| 关 键 词： | 胶体金，狂犬病病毒，中和抗体，CVS11 |
| 收稿时间： | 2010/12/22 0:00:00 |
Preparation and application of a colloidal gold strip to detect the rabies antibody |
|
| Tiecheng Wang,Tao Zhang,Songtao Yang,Hualei Wang,Yuwei Gao,Wei Sun,Xiaoxia Jin,Pingsen Zhao,Na Feng,Geng Huang,Xiaohuan Zou and Xianzhu Xia.Preparation and application of a colloidal gold strip to detect the rabies antibody[J].Chinese Journal of Biotechnology,2011,27(5):799-804. | |
| Authors: | Tiecheng Wang Tao Zhang Songtao Yang Hualei Wang Yuwei Gao Wei Sun Xiaoxia Jin Pingsen Zhao Na Feng Geng Huang Xiaohuan Zou and Xianzhu Xia |
| Institution: | Key Laboratory of Jilin Provice for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130122, China;Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China;Key Laboratory of Jilin Provice for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130122, China;College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China;Key Laboratory of Jilin Provice for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130122, China;College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China;Key Laboratory of Jilin Provice for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130122, China;Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China;Key Laboratory of Jilin Provice for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130122, China;Key Laboratory of Jilin Provice for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130122, China;Key Laboratory of Jilin Provice for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130122, China;Key Laboratory of Jilin Provice for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130122, China |
| Abstract: | To develop a specific,rapid,and convenient immunochromatography assay(ICA) to detect the rabies antibody in 800 ISSN1000-3061 CN11-1998/Q Chin J Biotech May 25,2011 Vol.27 No.5 clinical sample from immuned dogs by rabies vaccine.Colloidal gold particles labeled with purified rabies virus(CVS11) were used as the detector reagent.The staphylococcal protein A(SPA) and pured rabbit anti-rabies virus IgG were blotted on the test and control regions of nitrocellulose membrane.Then the strip was assembled with sam... |
| Keywords: | colloidal gold rabies virus neutralizing antibody CVS11 |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |
| 点击此处可从《生物工程学报》浏览原始摘要信息 | |
| 点击此处可从《生物工程学报》下载免费的PDF全文 | |