| 北极狐g-干扰素cDNA的克隆、表达及活性测定 |
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| 引用本文： | 张海玲,柴秀丽,罗国良,王凤雪,易立,邵西群,闫喜军.北极狐g-干扰素cDNA的克隆、表达及活性测定[J].生物工程学报,2008,24(9):1625-1630. |
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| 作者姓名： | 张海玲 柴秀丽 罗国良 王凤雪 易立 邵西群 闫喜军 |
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| 作者单位： | 中国农业科学院特产研究所, 吉林 132109;中国农业科学院特产研究所, 吉林 132109;中国农业科学院特产研究所, 吉林 132109;中国农业科学院特产研究所, 吉林 132109;中国农业科学院特产研究所, 吉林 132109;中国农业科学院特产研究所, 吉林 132109;中国农业科学院特产研究所, 吉林 132109 |
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| 基金项目： | 吉林省科技发展计划项目(No. 20060550)资助。 |
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| 摘 要： | 为研究狐g-干扰素的生物学活性, 应用反转录聚合酶链式反应(RT-PCR)从北极狐外周血淋巴细胞中扩增出g-干扰素(VuIFN-g)cDNA。序列分析表明VuIFN-g cDNA全长501 bp, 编码23个氨基酸的信号肽和144个氨基酸的成熟肽蛋白, 与已发表的银黑狐和犬IFN-g 核苷酸序列同源性为99.8%和99.4%; 氨基酸同源性均为100%。应用原核表达系统高效表达北极狐g-干扰素成熟肽蛋白, SDS-PAGE和Western blotting分析表达的融合蛋白分子量约为19 kD, 以不溶性的包涵体形式存在。重组蛋白经纯化和复性, 在Vero和MDCK细胞上可明显抑制VSV病毒的复制, 并测出北极狐重组g-干扰素的活性单位分别为1.0×106 u/mg和1.56×105 u/mg, 为进一步开发基因工程狐干扰素奠定基础。
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| 关 键 词： | 北极狐 干扰素-g 抗病毒活性 |
| 收稿时间： | 1/3/2008 12:00:00 AM |
Cloning, Expression and Antiviral Activity of Arctic Fox (Alopex lagopus) Interferon-g Gene |
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| Hailing Zhang,Xiuli Chai,Guoliang Luo,Fengxue Wang,Li Yi,Xiqun Shao and Xijun Yan.Cloning, Expression and Antiviral Activity of Arctic Fox (Alopex lagopus) Interferon-g Gene[J].Chinese Journal of Biotechnology,2008,24(9):1625-1630. |
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| Authors: | Hailing Zhang Xiuli Chai Guoliang Luo Fengxue Wang Li Yi Xiqun Shao and Xijun Yan |
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| Institution: | Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Jilin 132109, China;Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Jilin 132109, China;Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Jilin 132109, China;Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Jilin 132109, China;Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Jilin 132109, China;Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Jilin 132109, China;Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Jilin 132109, China |
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| Abstract: | In order to characterize the biological activity of fox (Vulpes vulpes) interferon gamma(VuIFN-g), We have isolated the cDNA encoding arctic fox(Alopex lagopus)VuIFN- g. This cDNA encodes a 23 amino acid signal peptide and a 144 amino acid mature protein, which shares 99.8% or 99.4% for nucleotide identity with silver fox and canine, respectively, and 100% for amino acid identity. Expression of recombinant mature arctic fox interferon gamma (mVuIFN-g) in bacterial system was confirmed by SDS-PAGE and Western blotting analysis. Recombinant VuIFN-g showed higher antiviral activity against vesicular stomatitis virus in cultured Vero and MDCK by inhibiting virus induced cytopathic effect, In view of the immunomodulatory and antiviral activities of VuIFN-g, it may provide a basis for further research on antiviral therapy of recombinant VuIFN-g in economic animal practice. |
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| Keywords: | Arctic fox interferon gamma antiviral activity |
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