| 腺病毒介导IL-24-E1A双基因载体的构建及其体外抑制SMMC-7721肝癌细胞生长作用初探 |
| |
| 引用本文： | 汪小华,缪竞诚,谢宇锋,盛伟华,单云波,杨吉成.腺病毒介导IL-24-E1A双基因载体的构建及其体外抑制SMMC-7721肝癌细胞生长作用初探[J].生物工程学报,2009,25(7):1035-1041. |
| |
| 作者姓名： | 汪小华 缪竞诚 谢宇锋 盛伟华 单云波 杨吉成 |
| |
| 作者单位： | 1. 苏州大学附属第一医院,苏州,215001
2. 苏州大学医学部基础医学与生物科学学院,细胞与分子生物学教研室,苏州,215123 |
| |
| 基金项目： | 江苏省高校自然科学研究项目(No.sz127822)资助~~ |
| |
| 摘 要： | 构建IL-24和E1A双基因腺病毒载体,获得Ad-IL-24-E1A重组腺病毒子,分析其体外抑瘤作用。PCR及BglⅡ和SalⅠ酶切获得IL-24,与空载体构建成pAdTrack-IL-24-IRES。XhoI和EcoRV酶切获得E1A片段,与pAdTrack-IL-24-IRES连接后成功构建pAdTrack-IL-24-IRES-E1A,同源重组、包装和扩增获得Ad-IL-24-E1A重组病毒子。用50MOI重组腺病毒感染SMMC-7721肝癌细胞,MTT法测定Ad-IL-24-E1A的细胞生长抑制作用;流式细胞仪分析细胞凋亡情况。本研究成功获得Ad-IL-24-E1A重组病毒子。与其他组相比,Ad-IL-24-E1A明显抑制肿瘤细胞生长,感染48h后凋亡率达52%,而抑制正常细胞作用不明显。研究提示Ad-IL-24-E1A双基因重组载体明显抑制SMMC-7721肝癌细胞生长。
|
| 关 键 词： | 构建 Ad-IL-24-E1A SMMC-7721肝癌细胞 抑瘤 |
| 收稿时间： | 2009/2/10 0:00:00 |
Construction of adenovirus vector expressing IL-24 and E1A and its inhibition of SMMC-7721 |
| |
| Xiaohua Wang,Jingcheng Miao,Yufeng Xie,Weihua Sheng,Yunbo Shan and Jicheng Yang.Construction of adenovirus vector expressing IL-24 and E1A and its inhibition of SMMC-7721[J].Chinese Journal of Biotechnology,2009,25(7):1035-1041. |
| |
| Authors: | Xiaohua Wang Jingcheng Miao Yufeng Xie Weihua Sheng Yunbo Shan and Jicheng Yang |
| |
| Institution: | No.1 Affiliated Hospital of Soochow University, Suzhou 215001, China;Department of Celluar and Molecular Biology, Medical School, Suzhou University, Suzhou 215123, China;Department of Celluar and Molecular Biology, Medical School, Suzhou University, Suzhou 215123, China;Department of Celluar and Molecular Biology, Medical School, Suzhou University, Suzhou 215123, China;Department of Celluar and Molecular Biology, Medical School, Suzhou University, Suzhou 215123, China;Department of Celluar and Molecular Biology, Medical School, Suzhou University, Suzhou 215123, China |
| |
| Abstract: | We constructed the recombinant adenovirus vector expressing IL-24 and E1A (Ad-IL-24-E1A) and investigated the inhibition of Ad-IL-24-E1A on SMMC-7721 hepatocellular carcinoma in vitro. We amplified IL-24 gene by PCR using pAdTrack-IL-24 as template. The IL-24 gene was cloned into pAdTrack-IRES at the Bgl II and Sal I site to form pAdTrack-IL-24-IRES. E1A digested from pAdTrack-E1A was cloned into the pAdTrack-IL-24-IRES at the Xho I and EcoR V site to form the pAdTrack-IL-24-IRES-E1A. We co-transformed both... |
| |
| Keywords: | construction Ad-IL-24-E1A SMMC-7721 growth-suppression |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《生物工程学报》浏览原始摘要信息 |
| 点击此处可从《生物工程学报》下载免费的PDF全文 |
|
