溶氧水平对红豆杉细胞悬浮培养的影响研究

紫杉醇 (Ｔａｘｏｌ)是源自红豆杉提取物的一种高度衍生化的二萜类化合物 ,临床实验结果表明紫杉醇对于卵巢癌、乳腺癌、胃肠道癌等具有明显的抗肿瘤活性1] ,因而受到世界各国的广泛关注 ,并已被美国食品与药品管理局 (ＦＤＡ)批准用于卵巢癌与乳腺癌的治疗2 ] 。到目前为止紫杉醇仍然主要从树皮中提取 ,但由于红豆杉生长缓慢 ,天然资源非常有限 ,加快其替代来源的研究势在必行。利用植物细胞悬浮培养生产紫杉醇作为一种可行的选择 ,近年来取得了较大的进展3 ,4 ] 。本文研究了摇瓶及 2 0 Ｌ反应器培养过程的溶氧水平对细胞生长及紫杉醇…

Effect of Dissolved Oxygen on the Suspension Culture of Taxus chinensis

The effect of dissolved oxygen controlled level on the taxol production by suspended cell culture of Taxus chinensis was examined in shake flasks and in a 20L aerated and agitated bioreactor with a six-flat-bladed turbine. It was found in shake flasks that oxygen supply exhibited significant influence on the production of taxol. Further investigation in bioreactor showed that noly 1.5 mg/L taxol was produced when DO(dissolved oxygen) level was 20%. Taxol production increased when DO level was increased to 40%-60%. Moreover, taxol accumulation was remarkably improved by the cultivation of cells initially at the DO level of 60% during the growth phase for 14 days followed by changing the DO level to 20%-40% during the taxol production phase for another 10 days. An optimum taxol level of 7.2 mg/L was obtained by the dissolved oxygen controlled process.