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| 水稻OsPLD3和OsPLD4基因及其启动子 | |
| 引用本文： | 杨 鹍,傅亚萍,张玉满,颜永胜,赵志强,方荣祥,孙宗修,陈晓英.水稻OsPLD3和OsPLD4基因及其启动子[J].生物工程学报,2008,24(3):368-375. |
| 作者姓名： | 杨 鹍 傅亚萍 张玉满 颜永胜 赵志强 方荣祥 孙宗修 陈晓英 |
| 作者单位： | 1. 中国科学院微生物研究所植物基因组学国家重点实验室,北京,100101;中国科学院研究生院,北京,100039 2. 中国水稻研究所水稻生物学国家重点实验室,杭州,310006 3. 中国科学院微生物研究所植物基因组学国家重点实验室,北京,100101 |
| 基金项目： | 中国科学院知识创新工程重要方向项目资助 (No. KSCXZ-YW-N-025)。 |
| 摘 要： | 磷脂酶D(Phospholipase D, PLD)是在植物组织中广泛存在的一类磷脂酶, 可催化磷脂如磷脂酰胆碱(phosphatidylcholine)水解产生磷脂酸(phosphatidic acid, PA)和一个自由的头部基团如胆碱(choline)。在植物体内PLD家族往往包括多个成员。利用反向遗传学技术对水稻磷脂酶D家族(OsPLD)中的两个成员OsPLD3和OsPLD4基因及其启动子的研究显示: OsPLD3和OsPLD4的启动子在花器官的不同部位中驱动报告基因不同程度地表达, 二者都受损伤和茉莉酸甲酯诱导, 但是对诱导因子反应的时空模式不同。利用转基因技术在水稻中过量表达OsPLD3和OsPLD4基因或是干扰OsPLD3和OsPLD4基因表达都不能引起可见的水稻表型的变化, 说明OsPLD家族不同成员可能有功能上的重复。 |
| 关 键 词： | 水稻磷脂酶D 损伤诱导 茉莉酸甲酯 花器官 |
| 收稿时间： | 2007-05-22 |
| 修稿时间： | 2007-06-14 |
Analysis of Rice OsPLD3 and OsPLD4 Genes and Promoters |
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| Kun Yang,Yaping Fu,Yuman Zhang,Yongsheng Yan,Zhiqiang Zhao,Rongxiang Fang,Zongxiu Sun and Xiaoying Chen.Analysis of Rice OsPLD3 and OsPLD4 Genes and Promoters[J].Chinese Journal of Biotechnology,2008,24(3):368-375. | |
| Authors: | Kun Yang Yaping Fu Yuman Zhang Yongsheng Yan Zhiqiang Zhao Rongxiang Fang Zongxiu Sun and Xiaoying Chen |
| Institution: | State Key Laboratory of plant genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China, Graduate School of Chinese Academy of Sciences, Beijing 100039, China;State Key Laboratory of Rice Biology, National Rice Research Institute, Hangzhou 310006, China;State Key Laboratory of plant genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;State Key Laboratory of plant genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China, Graduate School of Chinese Academy of Sciences, Beijing 100039, China;State Key Laboratory of plant genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China, Graduate School of Chinese Academy of Sciences, Beijing 100039, China;State Key Laboratory of plant genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;State Key Laboratory of Rice Biology, National Rice Research Institute, Hangzhou 310006, China;State Key Laboratory of plant genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China |
| Abstract: | Phospholipase Ds (PLDs) exist in many plants. PLDs catalyse the hydrolysis of phospholipids (e.g. phosphatidylcholine) in cell membrane into phosphatidic acid (PA) and polar free heads (e.g. choline). Two PLD members from rice, OsPLD3 and OsPLD4, were studied by reverse genetics approaches. The results showed that the promoters of OsPLD3 and OsPLD4 could drive the expression of the reporter gene in various tissues of the rice flower organs at different levels. The expression of both genes was induced by wounding and methyl jasmonate (MeJA), but with different intensity at different time intervals. No prominent phenotypes were observed by RNA interference with the gene-specific artificial miRNAs or over-expression of the target genes in rice plants, implying the functional redundancy among different members of the rice PLD family. |
| Keywords: | OsPLD wounding induction methyl jasmonate flower organ |
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