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| Spindle-view系统在猪卵母细胞去核中的应用 | |
| 引用本文： | 杨宇,戴建军,张廷宇,吴华莉,陈学进,张德福,马恒东.Spindle-view系统在猪卵母细胞去核中的应用[J].生物工程学报,2007,23(6):1140-1145. |
| 作者姓名： | 杨宇 戴建军 张廷宇 吴华莉 陈学进 张德福 马恒东 |
| 作者单位： | 1. 上海农业科学院畜牧兽医研究所,上海,201106;上海农业遗传育种重点实验室动物遗传工程研究室,上海,201106;四川农业大学动物科技学院,雅安,625014 2. 上海农业科学院畜牧兽医研究所,上海,201106;上海农业遗传育种重点实验室动物遗传工程研究室,上海,201106 3. 上海交通大学医学院上海发育生物学重点实验室,上海,200092 4. 四川农业大学动物科技学院,雅安,625014 |
| 基金项目： | 上海市科技兴农科技攻关项目 |
| 摘 要： | 应用Spindle-view对体外成熟培养36、42、44和48h的猪体外成熟卵母细胞减数分裂纺锤体进行去核操作,并与传统去核方法(McGrath-Solter去核法,挤压去核法)相比较,结果表明:①在42~48h之间利用Spindle-view得到的猪卵纺锤体影像与极体的相对位置没有明显的变化;②Spindle-view适合用于猪体外成熟卵母细胞减数分裂纺锤体的观察及去核;去核效率与其他两种方法相比差异极显著(95.5%,42.1%,74.2%,P<0.01);③纺锤体成像是否清晰可用于猪卵母细胞的质量监控。 |
| 关 键 词： | 猪卵母细胞 去核 减数分裂纺锤体 |
| 文章编号： | 1000-3061(2007)06-1140-06 |
| 修稿时间： | 2007年1月16日 |
Application of Spindle-view in the Enucleation Porcine of Oocytes |
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| YANG Yu,DAi Jian-Jun,ZHANG Ting-Yu,WU Hua-Li,CHEN Xue-Jin,ZHANG De-Fu and MA Heng-Dong.Application of Spindle-view in the Enucleation Porcine of Oocytes[J].Chinese Journal of Biotechnology,2007,23(6):1140-1145. | |
| Authors: | YANG Yu DAi Jian-Jun ZHANG Ting-Yu WU Hua-Li CHEN Xue-Jin ZHANG De-Fu and MA Heng-Dong |
| Institution: | Animal Husbandry and Veterinary Research Institude,Shanghai Academy of Agricultural Sciences, Shanghai 201106,China;Division of Animal Genetic Engineering,Shanghai Municipal Key Laboratory of Agri-genetic and Breeding,Shanghai 201106,;Animal Husbandry and Veterinary Research Institude,Shanghai Academy of Agricultural Sciences, Shanghai 201106,China;Division of Animal Genetic Engineering,Shanghai Municipal Key Laboratory of Agri-genetic and Breeding,Shanghai 201106,;Animal Husbandry and Veterinary Research Institude,Shanghai Academy of Agricultural Sciences, Shanghai 201106,China;Division of Animal Genetic Engineering,Shanghai Municipal Key Laboratory of Agri-genetic and Breeding,Shanghai 201106,;Animal Husbandry and Veterinary Research Institude,Shanghai Academy of Agricultural Sciences, Shanghai 201106,China;Division of Animal Genetic Engineering,Shanghai Municipal Key Laboratory of Agri-genetic and Breeding,Shanghai 201106,;Center of Developmental Biology, Xinhua Hospital, Shanghai Jiao Tong University, Shanghai 200092,China;Animal Husbandry and Veterinary Research Institude,Shanghai Academy of Agricultural Sciences, Shanghai 201106,China;Division of Animal Genetic Engineering,Shanghai Municipal Key Laboratory of Agri-genetic and Breeding,Shanghai 201106,;Animal Sci&Technology College, Sichuan Agriculture University,Ya'an 625014,China |
| Abstract: | The Spindle-view, a specialized instrument for observing spindle image, was applied to observe the meiotic spindles of vitro matured porcine oocytes at 36, 42, 44, 48h, and enucleation from porcine, comparing to the previously methods (McGrath-Solter's method and two-step-squeezing method) in the enucleated. The results showed that: (1) there was no noticeable differences at vicinity of spindle images and 1st polar body among in vitro matured porcine oocytes at 40-48 h under the instrument; (2) Spindle-view is suitable for the observation of meiotic spindles of matured oocytes and enucleation from porcine; the modified Spindle-view method for enucleation is significantly better than McGrath-Solter' s method and two-step-squeezing method in the enucleated rates (95.5%, 42.1%, 74.2%, P < 0.0l) of absolutely removing nuclei matter; (3) the spindle images could be used to monitor the oocyte qualities. |
| Keywords: | Spindle-view |
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