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圆红冬孢酵母菌发酵产油脂培养基及发酵条件的优化研究
引用本文:李永红,刘波,赵宗保,白凤武.圆红冬孢酵母菌发酵产油脂培养基及发酵条件的优化研究[J].生物工程学报,2006,22(4):650-656.
作者姓名:李永红  刘波  赵宗保  白凤武
作者单位:1. 大连理工大学生物科学与工程系,大连,116023
2. 中科院大连化学物理研究所生物技术部,大连,116023
3. 大连理工大学生物科学与工程系,大连,116023;中科院大连化学物理研究所生物技术部,大连,116023
基金项目:中国科学院百人计划项目;国家重点基础研究发展计划(973计划)
摘    要:采用均匀设计和单因子试验法,系统考察了圆红冬孢酵母菌(Rhodosporidiumtoruloides)在不同碳氮比条件下产油发酵情况以及添加无机盐对产油发酵的影响,通过均匀设计软件对二次多项回归方程求解及单因素分析得知在培养基组成分别为葡萄糖70g/L,硫酸铵0.1g/L,酵母粉0.75g/L,磷酸二氢钾0.4g/L,七水硫酸镁1.5g/L,初始pH6.0,在灭菌(121℃15min)后添加ZnSO41.91×10-6mmol/L、CaCl21.50mmol/L、MnCl21.22×10-4mmol/L、CuSO41.00×10-4mmol/L。发酵摇瓶装液量为250mL三角瓶装培养基50mL,接种量为10%(种龄28h)。在上述条件下,30℃振荡(200r/min)培养120h,所得菌体油脂含量高达76.1%,脂肪得率系数可达22.7。

关 键 词:圆红冬孢酵母菌  微生物油脂  发酵条件优化  均匀设计
文章编号:1000-3061(2006)04-0650-07
收稿时间:01 16 2006 12:00AM
修稿时间:04 11 2006 12:00AM

Optimized Culture Medium and Fermentation Conditions for Lipid Production by Rhodosporidium toruloides
LI Yong-Hong,LIU Bo,ZHAO Zong-Bao,BAI Feng-Wu.Optimized Culture Medium and Fermentation Conditions for Lipid Production by Rhodosporidium toruloides[J].Chinese Journal of Biotechnology,2006,22(4):650-656.
Authors:LI Yong-Hong  LIU Bo  ZHAO Zong-Bao  BAI Feng-Wu
Institution:Department of Bioscience and Bioengineering, Dalian University of Technology, Dalian 116023, China.
Abstract:Culture medium and fermentation conditions for lipid production by Rhodosporidium toruloides were optimized with single factor and uniform design experiment. The best medium recipe was found with 70 g/L glucose, 0.1 g/L (NH4)2SO4, 0.75 g/L yeast extract, 1.5 g/L MgSO4. 7H2O, 0.4g/L KH2PO4, sterilized at 121 degrees C for 15 min, and then supplemented with ZnSO4 1.91 x 10(-6) mmol/L, CaCl2 1.50 mmol/L, MnCl2 1.22 x 10(-4) mmol/L and CuSO4 1.00 x 10(-4) mmol/L. The optimal fermentation conditions were as follows: 50 mL of medium (pH 6.0) in 250 mL Erlenmeyer flask with 10% inoculum (28h) under orbital shaking at 200 r/min for 120h at 30 degrees C. Under these conditions, yeast biomass accumulated lipids up to 76.1%.
Keywords:Rhodosporidium toruloides  microbial lipids  culture optimization  uniform design  
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