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手性苯基环氧乙烷的生物不对称合成
引用本文:吴襟,程树华,沙倩,杨柳,孙万儒.手性苯基环氧乙烷的生物不对称合成[J].生物工程学报,2000,16(5):627-630.
作者姓名:吴襟  程树华  沙倩  杨柳  孙万儒
作者单位:中国科学院微生物研究所,微生物资源前期开发国家重点实验室,北京,100080
基金项目:国家自然科学基金支持的重大项目(29790128)。
摘    要:以苯乙烯为唯一碳源和能源,从不同来源的土壤样品中初筛分离出12株好氧细菌和2株真菌,经复筛,对液体培养物进行手性气相色谱分析,得到一株产生手性苯基环氧乙烷活力较高的菌种PS-1206,并对其发酵、产酶及苯乙烯的全细胞转化进行了研究,利用微生物细胞在30℃,pH 7.0,10mmol/L磷酸缓冲液中转化0.5%苯乙烯10h,获得?苯基环氧乙烷,e.e%值为80%,转化产率为35%。

关 键 词:环氧化酶,苯乙烯,手性苯基环氧乙烷,不对称生物合成
文章编号:1000-3061(2000)05-0627-04
修稿时间:2000-01-19

Asymmetric Biosynthesis of Chiral Styrene Oxide
WU Jin,CHENG Shu-Hua,SHA Qian,YANG Liu,SUN Wan-Ru.Asymmetric Biosynthesis of Chiral Styrene Oxide[J].Chinese Journal of Biotechnology,2000,16(5):627-630.
Authors:WU Jin  CHENG Shu-Hua  SHA Qian  YANG Liu  SUN Wan-Ru
Institution:WU Jin ,CHENG Shu-Hua ,SHA Qian ,YANG Liu ,SUN Wan-Ru ;(State Key Laboratory of Microbial Resources, Institute of Microbuiology, The Chinese Academy of Science, Beijing 100080)
Abstract:Used styrene as the sole carbon and energy source, 12 strains of aerobic bacterial and two strains of fungi were screened from a series soil samples. These strains were able to convert styrene to styrene oxide. HPLC and chiral GC method were used for determination the substrate, product and e.e value of culture broth, There were obvious effect on the biomass and epoxidase activity under various conditions in the fermentation. Styrene epoxidation of the strain PS-1206 was studied in different reaction time. 35% of conversion yield and 80% e.e value of (R)-styrene oxide could been obtained at the optimum conditions.
Keywords:Epoxidase  styrene  chiral styrene oxide  asymmetric biosynthesis
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