体外培养条件下SCF、LIF与bFGF对昆明白小鼠精原干细胞增殖的影响

生长因子作为细胞体外培养和在体细胞生长及增殖必需的调节因子 ,一直被广泛的关注。业已证明干细胞因子(ＳｔｅｍＣｅｌｌＦａｃｔｏｒ,ＳＣＦ)、白血病抑制因子 (ｌｅｕｋａｅｍｉａｉｎｈｉｂｉｔｏｒｙｆａｃｔｏｒ,ＬＩＦ)和碱性成纤维细胞生长因子 (ＢｓａｉｃＦｉｂｒｏｂｌａｓｔＧｒｏｗｔｈＦａｃｔｏｒ,ｂＦＧＦ)具有刺激细胞增殖的作用1～ 4] ,但大都是对单一因子进行研究。本实验探讨用这三种生长因子的不同组合观察对小鼠精原干细胞增殖的作用 ,以期定性和定量的探讨出体外培养初期三种因子对小鼠精原干细胞生长的影响 ,为小…

Effects of SCF, LIF and bFGF on Mouse Spermatogonial Stem Cells Proliferation in vitro

The present study identified the favorable environment conditions for Spermatogomial stem cells in vitro according to their unique biological properties. Three growth factors, stem cell factor (SCF), leukemia inhibitory factor (LIF) and basic fibroblast growth factor (bFGF) were all found to independently contribute to the proliferation of mouse spermatogonial stem cell. The percentage of cell proliferation significantly enhanced by SCF at 30 ng/mL but decreased with heightening its combination after cultured 120 hours. The mice spermatogonial stem cells were significantly proliferated after 120 hours' culture with 10 ng/mL and 20 ng/mL (P<0.01) of LIF, between 20 ng/mL and 50 ng/mL (P<0.01) for bFGF. SCF and bFGF were significantly enhanced mice spermatogonial stem cells proliferation after these three factors combination. For LIF, no obvious effect was abserved.