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| 去除选择标记基因的Cre/lox重组系统在植物中的应用 | |
| 引用本文： | 刘秀明,孟欣欣,李海燕,杨晶,付宏歧,李校堃.去除选择标记基因的Cre/lox重组系统在植物中的应用[J].生物工程学报,2009,25(10):1459-1463. |
| 作者姓名： | 刘秀明 孟欣欣 李海燕 杨晶 付宏歧 李校堃 |
| 作者单位： | 1. 吉林农业大学生物反应器与药物开发教育部工程研究中心,长春,130118 2. 吉林农业大学生物反应器与药物开发教育部工程研究中心,长春,130118;吉林农业大学生命科学学院,长春,130118 3. 吉林农业大学生物反应器与药物开发教育部工程研究中心,长春,130118;温州医学院,温州,325035 |
| 基金项目： | 国家高技术研究发展计划(863计划)(No.2007AA100503);;国家转基因生物新品种培育重大专项(No.2008ZX08010-002)资助~~ |
| 摘 要： | 获得无选择标记基因的转基因植物越来越受到研究者的重视。目前,应用得较广泛的去除选择标记基因的方法有共转化法和位点特异性重组法,其中位点特异性重组系统中Cre/lox重组系统研究最多。以下介绍了Cre/lox位点特异性重组系统的原理、特点及其近几年在植物中的应用,针对本实验室在这一领域的研究情况,重点阐述了Cre/lox系统的应用前景。随着植物反应器研究领域的不断壮大,去除筛选标记基因是植物反应器研究的必然趋势。 |
| 关 键 词： | 无选择标记基因 Cre/lox重组系统 转基因植物 |
| 收稿时间： | 2009/7/18 0:00:00 |
Application of the self excision Cre/lox system in plants |
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| Xiuming Liu,Xinxin Meng,Haiyan Li,Jing Yang,Hongqi Fu and Xiaokun Li.Application of the self excision Cre/lox system in plants[J].Chinese Journal of Biotechnology,2009,25(10):1459-1463. | |
| Authors: | Xiuming Liu Xinxin Meng Haiyan Li Jing Yang Hongqi Fu and Xiaokun Li |
| Institution: | Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China;Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China;Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China; College of Life Sciences, Jilin Agricultural University, Changchun 130118, China;Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China;Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China;Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China; School of Pharmaceutical Sciences, Wenzhou Medical College, Wenzhou 325035, China |
| Abstract: | Marker-free plants have been public concern. Co-transformation and site-specific recombination system are more important methods in self-gene excision. We reviewed the Cre/lox site-specific system and its applications in plants, also, we discussed perspectives of the system in according with our experience. |
| Keywords: | marker-free gene Cre/lox site-specific recombination system transgenic plant |
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