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| 利迪链霉菌A02抗真菌活性产物的分离和结构鉴定 | |
| 引用本文： | 隋勤,刘伟成,卢彩鸽,刘霆,裘季燕,刘学敏.利迪链霉菌A02抗真菌活性产物的分离和结构鉴定[J].生物工程学报,2009,25(6):840-846. |
| 作者姓名： | 隋勤 刘伟成 卢彩鸽 刘霆 裘季燕 刘学敏 |
| 作者单位： | 1. 北京市农林科学院植物保护环境保护研究所,北京,100097;东北农业大学植保系,哈尔滨,150030 2. 北京市农林科学院植物保护环境保护研究所,北京,100097 3. 东北农业大学植保系,哈尔滨,150030 |
| 基金项目： | 国家自然科学基金项目(No.30671346);;北京市自然科学基金项目(No.6072010);;北京市科技计划项目(No.D0706005040231)资助~~ |
| 摘 要： | 利迪链霉菌A02是从京郊森林土壤中分离筛选出的植物病原真菌高效拮抗菌株。为了明确其抑菌活性的物质基础,利用大孔树脂和硅胶吸附柱层析、HPLC循环制备分离等方法,从菌株A02发酵液中分离获得了纯度达99.845%以上的单一组分活性化合物。经紫外光谱、高分辨质谱、红外光谱和核磁共振谱的测定和解析,确定了该活性化合物的分子量为665,分子式为C33H47NO13,化学结构与四烯大环内酯类抗生素纳他霉素相同。这一结果揭示了利迪链霉菌产生抗真菌天然产物的新功能,并为纳他霉素在植物病害生物防治中的应用开拓了新的途径。 |
| 关 键 词： | 利迪链霉菌 活性产物 纳他霉素 病原真菌 |
| 收稿时间： | 2/9/2009 12:00:00 AM |
Extraction and structural identification of the antifungal metabolite of Streptomyces lydicus A02 |
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| Qin Sui,Weicheng Liu,Caige Lu,Ting Liu,Jiyan Qiu and Xuemin Liu.Extraction and structural identification of the antifungal metabolite of Streptomyces lydicus A02[J].Chinese Journal of Biotechnology,2009,25(6):840-846. | |
| Authors: | Qin Sui Weicheng Liu Caige Lu Ting Liu Jiyan Qiu and Xuemin Liu |
| Institution: | Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; Department of Plant Protection, Northeast Agricultural University, Harbin 150030, China;Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;Department of Plant Protection, Northeast Agricultural University, Harbin 150030, China |
| Abstract: | We isolated a high efficient antifungal strain A02 from forest soil in a suburb of Beijing.The result of polyphasic taxonomy confirmed that strain A02 belongs to Streptomyces lydicus.The fermented broth of the strain presented a stable and strong inhibiting activity against many plant pathogenic fungi.The purpose of this study was to ascertain the substance base of the antifungal activity of strain A02.We extracted the antifungal metabolite of A02 by using column chromatography with X-5 macroporous resin an... |
| Keywords: | Streptomyces lydicus antifungal metabolite natamycin pathogenic fungi |
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