| 大肠杆菌BL21(pTrc-gsh)与酵母耦联合成谷胱甘肽的研究 |
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| 引用本文： | 沈立新,魏东芝,张嗣良,王二力.大肠杆菌BL21(pTrc-gsh)与酵母耦联合成谷胱甘肽的研究[J].生物工程学报,2001,17(4):452-455. |
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| 作者姓名： | 沈立新 魏东芝 张嗣良 王二力 |
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| 作者单位： | [1]华东理工大学生物反应器工程工程国家重点实验室，生物化学研究所，上海200237 [2]华东理工大学生物反应器工程工 |
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| 摘 要： | 谷胱甘肽 (ＧＳＨ)广泛存在于动、植物和微生物细胞内 ,有参与氨基酸的跨膜运输、维持细胞的还原状态等重要生理功能 ,在临床、保健品、食品等行业有广泛用途 ,如 :重金属解毒、抗氧化延缓衰老等 ,我国基本靠进口。开发高效、低成本的ＧＳＨ生产工艺势在必行。谷胱甘肽的制备有化学合成法1 ] 、提取法2 ] 、微生物发酵法3] 、酶法4] 等。由于酶法合成ＧＳＨ的产率高、后续的分离提取较简单而倍受关注。它是以ＡＴＰ为能量供体、由γ 谷氨酰半胱氨酸合成酶 (ＧＳＨＩ)和谷胱甘肽合成酶 (ＧＳＨＩＩ)连续催化合成的 :谷氨酸 半胱氨酸 Ａ…
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| 关 键 词： | 谷胱甘肽 ATP 工程菌 酵母 大肠杆菌 合成 |
| 文章编号： | 1000-3061(2001)04-0452-04 |
| 修稿时间： | 2000年12月21 |
Studies on Synthesis of Glutathione by E. coliBL21 (pTrc-gsh) Coupled with Saccharomyces cerevisiae |
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| SHEN Li-Xin\ WEI Dong-Zhi,ZHANG Si-Liang\ WANG Er-Li.Studies on Synthesis of Glutathione by E. coliBL21 (pTrc-gsh) Coupled with Saccharomyces cerevisiae[J].Chinese Journal of Biotechnology,2001,17(4):452-455. |
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| Authors: | SHEN Li-Xin\ WEI Dong-Zhi ZHANG Si-Liang\ WANG Er-Li |
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| Institution: | State Key Laboratory of Bioreactor Engineering, Institute of Biochemistry, ECUST, Shanghai 200237, China. |
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| Abstract: | During synthesis of GSH by the engineered strain E.coli BL21(pTrc-gsh) coupled with Saccharomyces cerevisiae producing ATP from adenosin,the inconsistency of two systems in the concentration of phosphate buffer was solved by decreasing concentration to 250mmol/L.The conditions under 250mmol/L phosphate buffer were optimized and the yield of GSH was 1.6g/L,which was higher than that of summation by two systems under the same conditions respectively.Addition of glycine later after glutamate and cysteine weakened the inhibition of GSH to GSHI.It made the yield of GSH reach to 2.13g/L which was 30.7% higher than the control. |
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| Keywords: | glutathione synthesis engineered strain S cerevisiae coupled |
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