| 初始底物浓度及pH对丁酸梭菌T4发酵木糖产氢的影响 |
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| 引用本文： | 邱颉,许继飞,任南琪.初始底物浓度及pH对丁酸梭菌T4发酵木糖产氢的影响[J].生物工程学报,2009,25(6):887-891. |
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| 作者姓名： | 邱颉 许继飞 任南琪 |
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| 作者单位： | 哈尔滨工业大学,城市水资源与水环境国家重点实验室,哈尔滨,150090 |
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| 基金项目： | 国家自然科学基金项目(No.30470054)资助~~ |
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| 摘 要： | 本研究采用间歇培养方式对丁酸梭菌T4发酵木糖进行产氢研究,考察初始pH和初始底物浓度对其产氢特性的影响。结果表明,菌株T4在初始pH5.0～8.5及初始底物浓度5～40g/L时均可以产氢,其累积产氢量和最大比产氢速率随着pH及底物浓度的增加均呈现先增加后减少的趋势。在pH6.5和底物浓度20g/L时,比产氢速率和累积产氢量达到最大,分别为4.26L/L和18.86mmol-H2/hg-DCW,而后随着pH或者底物浓度的增加二者均呈现减少的趋势;在pH6.5和底物浓度15g/L时,得到最大值比产氢量为2.17mol/mol-木糖。而在不同的pH下,发酵产生的液态产物主要是乙酸和丁酸,其中在pH小于6.0时,有少量的丙酸生成,而在pH大于6.0时,则有乙醇生成。
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| 关 键 词： | 发酵产氢 丁酸梭菌T4 木糖 初始pH 底物浓度 |
| 收稿时间： | 2009/3/15 0:00:00 |
Effect of initial substrate concentrations and pH on hydrogen production from xylose with Clostridium butyricum T4 |
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| Jie Qiu,Jifei Xu and Nanqi Ren.Effect of initial substrate concentrations and pH on hydrogen production from xylose with Clostridium butyricum T4[J].Chinese Journal of Biotechnology,2009,25(6):887-891. |
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| Authors: | Jie Qiu Jifei Xu and Nanqi Ren |
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| Institution: | State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China;State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China;State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China |
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| Abstract: | We studied the effect of initial pH and substrate concentrations on the conversion of xylose to hydrogen by Clostridium butyrium T4 at pH 5.0-8.5 and substrate concentrations 5?40 g/L.The cumulative hydrogen volume and the specific hydrogen production rate increased and then decreased with increasing initial pH or substrate concentrations.At initial pH 6.5 and substrate concentration 20 g/L,the cumulative hydrogen production and the specific hydrogen production rate reached the maximum value of 4.26 L/L and... |
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| Keywords: | fermentative hydrogen Clostridium butyrium T4 xylose initial pH substrate concentration |
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