| spt15突变基因对酿酒酵母木糖利用的影响 |
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| 引用本文： | 刘红梅,唐雯,来灿钢,严明,许琳,欧阳平凯.spt15突变基因对酿酒酵母木糖利用的影响[J].生物工程学报,2009,25(6):875-879. |
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| 作者姓名： | 刘红梅 唐雯 来灿钢 严明 许琳 欧阳平凯 |
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| 作者单位： | 南京工业大学制药与生命科学学院,材料化学工程国家重点实验室,南京,210009 |
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| 基金项目： | 国家自然科学基金-广东联合项目(No.U0733001);;南京工业大学博士论文创新基金(No.BSCX200810)资助~~ |
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| 摘 要： | 利用基因工程手段得到重组菌YPH499-3中的spt15有效突变基因,通过表达载体pYX212转化入酿酒酵母原始菌株YPH499中,重新获得酿酒酵母重组菌株。对其性状进行研究,结果表明该菌株能有效利用木糖并共发酵木糖和葡萄糖。在30oC、200r/min,发酵72h时,50g/L木糖的利用率为82.0%,乙醇产率为28.4%;当木糖和葡萄糖以质量比1:1混合发酵时,木糖和葡萄糖的利用率分别为80.4%和100%,乙醇产率为31.4%;同时发现木糖醇的含量极低。从而验证了有效突变基因spt15-10对酿酒酵母共发酵木糖和葡萄糖产酒精的影响。
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| 关 键 词： | spt15 酿酒酵母 木糖利用 共发酵 |
| 收稿时间： | 3/3/2009 12:00:00 AM |
Effects of mutational spt15 gene to xylose utilization of Saccharomyces cerevisiae |
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| Hongmei Liu,Wen Tang,Cangang Lai,Ming Yan,Lin Xu and Pingkai Ouyang.Effects of mutational spt15 gene to xylose utilization of Saccharomyces cerevisiae[J].Chinese Journal of Biotechnology,2009,25(6):875-879. |
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| Authors: | Hongmei Liu Wen Tang Cangang Lai Ming Yan Lin Xu and Pingkai Ouyang |
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| Institution: | State Key Laboratory of Materials-oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China;State Key Laboratory of Materials-oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China;State Key Laboratory of Materials-oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China;State Key Laboratory of Materials-oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China;State Key Laboratory of Materials-oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China;State Key Laboratory of Materials-oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China |
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| Abstract: | |
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| Keywords: | spt15 Saccharomyces cerevisiae xylose utilization co-fermentation |
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