| 根癌农杆菌介导的黄瓜转基因研究进展 |
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| 引用本文： | 柴里昂,樊怀福,刘晨,杜长霞.根癌农杆菌介导的黄瓜转基因研究进展[J].生物工程学报,2020,36(4):643-651. |
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| 作者姓名： | 柴里昂 樊怀福 刘晨 杜长霞 |
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| 作者单位： | 浙江农林大学 农业与食品科学学院 浙江省农产品品质改良技术研究重点实验室，浙江 杭州 311300 |
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| 基金项目： | 浙江省自然科学基金 (Nos. LY18C150004，LY18C150003，LY15C150006) 资助。 |
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| 摘 要： | 黄瓜Cucumis sativus是世界性的重要蔬菜作物。农杆菌介导的转基因技术是研究植物基因功能及品种改良的重要手段。为进一步加快黄瓜的转基因研究和育种进程,文中针对农杆菌介导的黄瓜遗传转化方法,从黄瓜再生能力的影响因素、遗传转化条件和过程中各类添加物质等方面,阐述了根癌农杆菌介导的黄瓜转基因研究进展及存在的问题,并对提高黄瓜遗传转化效率和安全筛选标记的应用等前景进行了展望,以期为黄瓜抗逆育种和果实品质改良等研究提供参考。
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| 关 键 词： | 黄瓜 转基因 再生能力 遗传转化效率 |
| 收稿时间： | 2019/6/20 0:00:00 |
Advances in Agrobacterium tumefaciens-mediated transgenic cucumber |
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| Li’ang Chai,Huaifu Fan,Chen Liu,Changxia Du.Advances in Agrobacterium tumefaciens-mediated transgenic cucumber[J].Chinese Journal of Biotechnology,2020,36(4):643-651. |
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| Authors: | Li’ang Chai Huaifu Fan Chen Liu Changxia Du |
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| Institution: | Zhejiang Provincial Key Laboratory of Agricultural Product Quality Improvement Technology, College of Agriculture and Food Science, Zhejiang A&F University, Hangzhou 311300, Zhejiang, China |
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| Abstract: | Cucumber (Cucumis sativus) is an important vegetable crop in the world. Agrobacterium-mediated transgenic technology is an important way to study plant gene functions and improve varieties. In order to further accelerate the transgenic research and breeding process of cucumber, we described the progress and problems of Agrobacterium tumefaciens-mediated transgenic cucumber, from the influencing factors of cucumber regeneration ability, genetic transformation conditions and various additives in the process. We prospected for improving the genetic transformation efficiency and safety selection markers of cucumber, and hoped to provide reference for the research of cucumber resistance breeding and quality improvement. |
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| Keywords: | cucumber transgenic regenerative capacity genetic transformation efficiency |
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