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虹鳟生长激素cDNA在酵母中的表达
引用本文:马进,白俊杰.虹鳟生长激素cDNA在酵母中的表达[J].生物工程学报,1999,15(4):434-438.
作者姓名:马进  白俊杰
作者单位:珠江水产研究所农业部热带亚热带鱼类选育与养殖重点开,珠江水产研究所农业部热带亚热带鱼类选育与养殖重点开放实验室!广州510380,珠江水产研究所农业部热带亚热带鱼类选育与养殖重点开放实验室!广州510380,珠江水产研究所农业部热带亚热带鱼类选育与养殖重点开放实验室!广州5
基金项目:农业部“九五”渔业科技重点项目,中国水产科学研究院基金
摘    要:采用聚合酶链式反应( P C R) 技术对虹鳟生长激素c D N A 进行改造。将改造后的基因克隆到含酵母 P G K 启动子的大肠杆菌酵母穿梭质粒p M A91 ,转化酿酒酵母 Y33 ,构建表达鱼生长激素的酵母工程菌 Y33(p M Ar G H16) ,并在酵母中获得表达,表达量约占细胞可溶性蛋白总量的3 % 。表达产物作为饲料添加剂投喂罗非鱼,具有明显的促进生长作用

关 键 词:虹鳟生长激素  酵母  基因表达  口服  促生长

Expression of Rainbow Trout Growth Hormone cDNA in Yeast Saccharomyces cerevisiae Suppored by Key Project on Fish Science and Technology of Agriculture Ministry (No.95 B 96 02 02 04),and the Fund of CAFS (No.95 08 01).
Ma Jin,Bai Junjie,Li Xinhui,Luo Jianren,Jian Qing,Zhang Hongjun.Expression of Rainbow Trout Growth Hormone cDNA in Yeast Saccharomyces cerevisiae Suppored by Key Project on Fish Science and Technology of Agriculture Ministry (No.95 B 96 02 02 04),and the Fund of CAFS (No.95 08 01).[J].Chinese Journal of Biotechnology,1999,15(4):434-438.
Authors:Ma Jin  Bai Junjie  Li Xinhui  Luo Jianren  Jian Qing  Zhang Hongjun
Abstract:Rainbow trout growth hormone cDNA was modified by PCR.Then the cDNA was subcloned into the expression vector pMA91,and transformed into yeast Saccharomyces cerevisiae Y33 to construct an expression strain Y33(pMArGH16).The recombinant gene could highly express the growth hormone peptide (about 3% of the total proteins) in Y33 (pMArGH16).The expression product was used as a supplement to feed Tilapia fingerling.The result showed that the recombinant fish GH has notable growth enhancement to Tilapia fingerling.
Keywords:Rainbow trout growth hormone  gene expression in yeast  take orally  growth enhancement  
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