| hNOK抗体的制备及肺癌组织中NOK的表达检测 |
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| 引用本文： | 徐方,李颖华,王银银,任芳丽,刘力,常智杰.hNOK抗体的制备及肺癌组织中NOK的表达检测[J].生物工程学报,2008,24(3):480-484. |
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| 作者姓名： | 徐方 李颖华 王银银 任芳丽 刘力 常智杰 |
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| 作者单位： | 1. 清华大学医学院,清华大学生物科学与技术系,生物膜与膜生物工程国家重点实验室,北京,100084
2. 中国协和医科大学基础医学院,中国医学科学院基础医学研究所,北京,100005 |
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| 基金项目： | 国家自然科学基金(No. 30530420, 30611120522), 国家支撑计划(No. 2006CB910100), 清华裕元基金(No. 202400005-03, 202400005-06)资助。 |
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| 摘 要： | Novel Oncogene with Kinase-domain (NOK)是一个新的肿瘤相关基因, 其结构上?与受体蛋白相似, 具有一个蛋白激酶结构域。过量表达NOK会导致肿瘤的产生与转移。为了更进一步研究生理状态下NOK的功能, 需要制备NOK特异性的抗体。利用GST融合蛋白制备了人源NOK的多克隆抗体, 并进行了纯化。检测表明所得的抗体具有很高的滴度, 能够特异性检测NOK蛋白的表达。使用该抗体进行了人肺癌组织的免疫组化检测, 发现该抗体能够高效识别组织内源性表达的NOK蛋白, 可为肺癌的诊断提供依据。
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| 关 键 词： | 蛋白激酶 多克隆抗体 NOK 肺癌 |
| 收稿时间： | 2006-06-21 |
| 修稿时间： | 2007-11-14 |
Preparation of Anti-hNOK Antibodies and Expression Examination of NOK in the Lung Cancer Tissues |
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| Fang Xu,Yinghua Li,Yinyin Wang,Fangli Ren,Li Liu and Zhijie Chang.Preparation of Anti-hNOK Antibodies and Expression Examination of NOK in the Lung Cancer Tissues[J].Chinese Journal of Biotechnology,2008,24(3):480-484. |
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| Authors: | Fang Xu Yinghua Li Yinyin Wang Fangli Ren Li Liu and Zhijie Chang |
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| Institution: | State Key Laboratory of Biomembrane and Membrane Biotechnology, School of Medicine, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;State Key Laboratory of Biomembrane and Membrane Biotechnology, School of Medicine, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;State Key Laboratory of Biomembrane and Membrane Biotechnology, School of Medicine, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;State Key Laboratory of Biomembrane and Membrane Biotechnology, School of Medicine, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;Department of Microbiology and Etiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China;State Key Laboratory of Biomembrane and Membrane Biotechnology, School of Medicine, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China |
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| Abstract: | Novel Oncogene with Kinase-domain (NOK) is a novel tumor-related gene, coding receptor like protein with a kinase domain. Overexpression of NOK leads to tumorigenesis and metastasis. To further study NOK function in physiological condition, it is necessary to prepare the anti-NOK antibody. In this report, GST fusion protein was adopted to prepare polyclonal antibodies against hNOK. The result showed that the antibodies we generated is with a very high titriation, and can be used for examination of NOK protein by Westernblot. Furthermore, the antibodies were used for immunohistochemistry in lung cancer tissues, and the results demonstrated high expression of hNOK in the tumor tissues. The antibody of hNOK we generated can serve as a diagnostic method for the lung cancer. |
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| Keywords: | kinase polyclonal antibody NOK lung cancer |
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