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| 分阶段pH调控提高2-酮基-L-古龙酸生产 | |
| 引用本文： | 张静,周景文,刘立明,刘杰,陈克杰,堵国成,陈坚.分阶段pH调控提高2-酮基-L-古龙酸生产[J].生物工程学报,2010,26(9):1263-1268. |
| 作者姓名： | 张静 周景文 刘立明 刘杰 陈克杰 堵国成 陈坚 |
| 作者单位： | 1. 江南大学,工业生物技术教育部重点实验室,无锡,214122;江南大学生物工程学院,生物系统与生物加工工程研究室,无锡,214122 2. 江苏江山制药有限公司,靖江,214500 3. 江南大学生物工程学院,生物系统与生物加工工程研究室,无锡,214122 |
| 基金项目： | 国家杰出青年基金 (No. 20625619)，国家重点基础研究发展计划 (973计划) (No. 2007CB 714306)，江苏高等学校优秀科技创新团队项目，国家高技术研究发展计划 (863计划) (No. 2006AA020303)，“十一五”国家科技支撑计划 (No. 2007BAI46B02) 资助。 |
| 摘 要： | 为了提高酮古龙酸菌Ketogulonicigenium vulgare和巨大芽胞杆菌Bacillus megaterium生产2-酮基-L-古龙酸(2-KLG)的生产效率,分析了pH对K.vulgare和B.megaterium生长和产酸的影响,发现K.vulgare和B.megaterium的最适生长pH值分别为6.0和8.0,但是K.vulgare的糖酸转化活力在pH7.0时达到最大值,因此提出了三阶段pH控制策略(第一阶段:0～8h,pH8.0;第二阶段:8～20h,pH6.0;第三阶段:20h至发酵结束,pH7.0)以促进K.vulgare生长和2-KLG生产。结果表明,三阶段pH控制策略的实施进一步提高了2-KLG的产量(77.3g/L)、生产强度(1.38g/(L·h))和L-山梨糖消耗速率(1.42g/(L·h)),分别比恒定pH7.0时提高了9.7%、33.2%和25.7%。 |
| 关 键 词： | pH调控策略，酮古龙酸菌，巨大芽胞杆菌 |
| 收稿时间： | 2010/1/29 0:00:00 |
Enhancement of 2-keto-L-gulonic acid production using three-stage pH control strategy |
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| Jing Zhang,Jingwen Zhou,Liming Liu,Jie Liu,Kejie Chen,Guocheng Du and Jian Chen.Enhancement of 2-keto-L-gulonic acid production using three-stage pH control strategy[J].Chinese Journal of Biotechnology,2010,26(9):1263-1268. | |
| Authors: | Jing Zhang Jingwen Zhou Liming Liu Jie Liu Kejie Chen Guocheng Du and Jian Chen |
| Institution: | Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Biological Systems and Bio-processing Laboratory, School of Biotechnology, Jiangnan University, Wuxi 214122, China;Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Biological Systems and Bio-processing Laboratory, School of Biotechnology, Jiangnan University, Wuxi 214122, China;Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Biological Systems and Bio-processing Laboratory, School of Biotechnology, Jiangnan University, Wuxi 214122, China;Jiangsu Jiangshan Pharmaceutical Co., Ltd., Jingjiang 214500, China;Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Biological Systems and Bio-processing Laboratory, School of Biotechnology, Jiangnan University, Wuxi 214122, China;Biological Systems and Bio-processing Laboratory, School of Biotechnology, Jiangnan University, Wuxi 214122, China;Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Biological Systems and Bio-processing Laboratory, School of Biotechnology, Jiangnan University, Wuxi 214122, China |
| Abstract: | The aim of this study was to improve the 2-keto-L-gulonic acid (2-KLG) production efficiency by Ketogulonicigenium vulgare and Bacillus megaterium by using multi-stage pH control strategy. The effect of pH on the cell growths and 2-KLG production showed that the optimum pH for K. vulgare and B. megaterium cell growth were 6.0 and 8.0, respectively, while the optimum pH for 2-KLG production was 7.0. Based on the above results, we developed a three-stage pH control strategy: the pH was kept at 8.0 during the first 8 h, then decreased to 6.0 for the following 12 h, and maintained at 7.0 to the end of fermentation. With this strategy, the titer, productivity of 2-KLG and L-sorbose consumption rate were achieved at 77.3 g/L, 1.38 g/(L·h) and 1.42 g/(L·h), respectively, which were 9.7%, 33.2% and 25.7% higher than the corresponding values of the single pH (pH 7.0) control model. |
| Keywords: | pH control strategy Ketogulonicigenium vulgare Bacillus megaterium |
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