| 不同佐剂对含S+PreS1融合抗原的乙型肝炎病毒颗粒疫苗免疫原性的影响 |
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| 引用本文： | 陈红,邓瑶,谭文杰,王文,殷霄,管洁,王文玲,阮力.不同佐剂对含S+PreS1融合抗原的乙型肝炎病毒颗粒疫苗免疫原性的影响[J].生物工程学报,2010,26(1):74-78. |
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| 作者姓名： | 陈红 邓瑶 谭文杰 王文 殷霄 管洁 王文玲 阮力 |
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| 作者单位： | 中国疾病预防控制中心病毒病预防控制所病毒病应急技术中心; |
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| 基金项目： | 重大新药创制科技重大专项(No.2009ZX09102-237)资助~~ |
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| 摘 要： | 本研究在前期工作基础上,用CHO细胞表达的含PreS1+S融合抗原的新型基因工程HBV颗粒疫苗(HBSS1)与Al(OH)3、CpG及CpG+Al(OH)3等佐剂配伍,在Balb/C小鼠模型上研究不同佐剂对HBV颗粒疫苗肌肉注射后免疫应答的影响,主要包括抗体滴度、抗体亚型分类及特异性细胞免疫(γ-IFNELISpot检测)。结果表明:CpG佐剂结合HBSS1颗粒疫苗可快速诱导(单针免疫)高水平的抗PreS1及S抗体,IgG2a/IgG1比率1,同时可诱导较高抗原特异的细胞免疫应答;Al(OH)3+CpG双佐剂组一次免疫后可诱导产生最高的抗S抗体滴度(1:105),其产生的抗体亚类包括IgG1、IgG2a与IgG2b;在S抗原N端(13~49aa)存在优势CTL表位。结论:CpG佐剂结合HBSS1颗粒疫苗应是发展新型治疗性乙肝疫苗的较佳选项。
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| 关 键 词： | 乙型肝炎病毒 病毒颗粒 疫苗 CpG佐剂 |
| 收稿时间： | 2009/8/16 0:00:00 |
Impact of different adjuvant on immunogenicity of the HBV particle vaccine containing the S + PreS1 fusion antigen in Balb/C mice |
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| Hong Chen,Yao Deng,Wenjie Tan,Wen Wang,Xiao Yin,Jie Guan,Wenling Wang and Li Ruan.Impact of different adjuvant on immunogenicity of the HBV particle vaccine containing the S + PreS1 fusion antigen in Balb/C mice[J].Chinese Journal of Biotechnology,2010,26(1):74-78. |
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| Authors: | Hong Chen Yao Deng Wenjie Tan Wen Wang Xiao Yin Jie Guan Wenling Wang and Li Ruan |
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| Institution: | Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China;Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China;Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China;Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China;Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China;Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China;Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China;Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China |
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| Abstract: | We previous reported the development of novel hepatitis B virus(HBV) vaccine containing the surface antigen(S) plus PreS1 fusion derived from Chinese hamster ovary (CHO) cells system. In this study, we analyzed the impact of different adjuvants on immunogenicity of the HBV particle vaccine in Balb/C mice, including alum alone, CpG oligodeoxynucleotides (CpG-ODN) alone and CpG-ODN in combination with alum adjuvant. We first detected the antigen specific humoral response in mice, including total IgG antibody ... |
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| Keywords: | hepatitis B virus (HBV) viral particles vaccine CpG-ODN |
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