| 人类博卡病毒HBoV1基因组克隆及启动子活性分析 |
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| 引用本文： | 李京京,孙彬,欧阳锦凤,陈莹,韩虎,刘凯于,李毅.人类博卡病毒HBoV1基因组克隆及启动子活性分析[J].生物工程学报,2011,27(6):909-916. |
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| 作者姓名： | 李京京 孙彬 欧阳锦凤 陈莹 韩虎 刘凯于 李毅 |
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| 作者单位： | 1. 华中师范大学生命科学学院,武汉430079;湖北师范学院生命科学学院,黄石435002
2. 华中师范大学生命科学学院,武汉,430079 |
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| 基金项目： | 国家自然科学基金 (No. 30670081) 资助。 |
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| 摘 要： | 人类博卡病毒 (Human bocavirus，HBoV) 是继细小病毒B19之后，第2个被发现可引起人类疾病的细小病毒。通过PCR扩增方法从患有下呼吸道感染的患儿痰液中鉴定HBoV，以鉴定的阳性样本为模板，利用分子生物学方法构建病毒基因组克隆并进行序列分析。2007年10月?2009年3月从湖北省妇幼保健院共收集941例下呼吸道感染患儿的痰液标本，检测到33份HBoV阳性样品，阳性率为3.51％ (33/941)；其中1岁以下婴幼儿患者占阳性样72.7％；构建了含有HBoV中间大片段基因克隆WHL-1，
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| 关 键 词： | 人类博卡病毒 (HboV1)，克隆，序列分析，启动子活性 |
| 收稿时间： | 2010/9/27 0:00:00 |
Genome cloning of human bocavirus (HBoV1) and analysis of viral promoter activity |
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| Jingjing Li,Bin Sun,Jinfeng Ouyang,Ying Chen,Hu Han,Kaiyu Liu and Yi Li.Genome cloning of human bocavirus (HBoV1) and analysis of viral promoter activity[J].Chinese Journal of Biotechnology,2011,27(6):909-916. |
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| Authors: | Jingjing Li Bin Sun Jinfeng Ouyang Ying Chen Hu Han Kaiyu Liu and Yi Li |
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| Institution: | College of Life Science, Central China Normal University, Wuhan 430079, China; College of Life Science, Hubei Normal University, Huangshi 435002, China;College of Life Science, Central China Normal University, Wuhan 430079, China;College of Life Science, Central China Normal University, Wuhan 430079, China;College of Life Science, Central China Normal University, Wuhan 430079, China;College of Life Science, Central China Normal University, Wuhan 430079, China;College of Life Science, Central China Normal University, Wuhan 430079, China;College of Life Science, Central China Normal University, Wuhan 430079, China |
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| Abstract: | Human bocavirus(HBoV) is a recently discovered parvovirus,which is suspected to be an etiologic agent of respiratory disease and gastrointestinal disease in human.In the present study,we screened 941 nasopharyngeal aspirates collected from hospitalized children with lower respiratory tract infections from October 9,2007 to March 20,2009 in the Children's Hospital of Hubei Province.Our results showed that 33 of 941 samples(3.51%) were detected positive for HBoV.To obtain a full-length HBoV clone,three segmen... |
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| Keywords: | human bocavirus (HBoV1) cloning sequence analysis promoter activity |
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