| 激光显微切割/质谱联用技术在肾脏疾病诊断中的应用进展 |
| |
| 引用本文： | 孙颖,李明喜,文煜冰,李雪梅,孙健,孙伟.激光显微切割/质谱联用技术在肾脏疾病诊断中的应用进展[J].生物工程学报,2014,30(7):1134-1140. |
| |
| 作者姓名： | 孙颖 李明喜 文煜冰 李雪梅 孙健 孙伟 |
| |
| 作者单位： | 1 中国医学科学院 北京协和医学院 北京协和医院 肾内科，北京 100730;1 中国医学科学院 北京协和医学院 北京协和医院 肾内科，北京 100730;1 中国医学科学院 北京协和医学院 北京协和医院 肾内科，北京 100730;1 中国医学科学院 北京协和医学院 北京协和医院 肾内科，北京 100730;2 中国医学科学院 北京协和医学院 北京协和医院 病理科，北京 100730;3 中国医学科学院 北京协和医学院 基础医学研究所 中心仪器室，北京 100730 |
| |
| 基金项目： | 北京协和医院青年科研基金 (No. 20132755)，“十二五”国家科技支撑计划 (Nos. 2011BA110B00, 2011BA110B05)，卫生公益行业科研专项基金(No. 201002010)，诺和诺德协和糖尿病英才研究基金 (2014年) 资助。 |
| |
| 摘 要： | 激光显微切割(Laser microdissection,LMD)质谱(Mass spectrometry,MS)联用技术(LMD/MS)已成功应用于肾活检组织甲醛固定石蜡包埋切片的蛋白质组学研究,提高了某些肾脏病的诊断水平,显示出较好的临床应用前景。文中就LMD/MS蛋白质组学技术的原理、方法及该技术在肾淀粉样变性、膜增殖性肾小球肾炎等肾脏疾病的发病机制及诊断分型的应用进展进行综述。
|
| 关 键 词： | 激光显微切割技术 甲醛固定石蜡包埋组织蛋白质组 肾淀粉样变性 膜增殖性肾小球肾炎 |
| 收稿时间： | 2014/2/22 0:00:00 |
Laser microdissection and mass spectrometry based proteomics in the diagnosis of kidney diseases |
| |
| Ying Sun,Mingxi Li,Yibing Wen,Xuemei Li,Jian Sun and Wei Sun.Laser microdissection and mass spectrometry based proteomics in the diagnosis of kidney diseases[J].Chinese Journal of Biotechnology,2014,30(7):1134-1140. |
| |
| Authors: | Ying Sun Mingxi Li Yibing Wen Xuemei Li Jian Sun and Wei Sun |
| |
| Institution: | 1 Department of Nephrology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China;1 Department of Nephrology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China;1 Department of Nephrology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China;1 Department of Nephrology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China;2 Department of Pathology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China;3 Department of Core Instrument Facility, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100730, China |
| |
| Abstract: | In recent years, laser microdissection followed by mass spectrometry (LMD/MS) has been successfully applied to the proteomic studies of formalin-fixed paraffin-embedded (FFPE) renal tissues. This new technique improves the diagnosis of kidney diseases and has a better potential for future clinical application. The review focuses on the use of this methodology for exploring the mechanisms, diagnosis and classification of kidney diseases including renal amyloidosis and membrane proliferative glomerulonephritis. |
| |
| Keywords: | laser microdissection FFPE proteomics renal amyloidosis membrane proliferative glomerulonephritis |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《生物工程学报》浏览原始摘要信息 |
| 点击此处可从《生物工程学报》下载免费的PDF全文 |
|
