甘蓝下胚轴原生质体再生植株

经纯化后，甘蓝下胚轴原生质体的产量为1．5×10⁶g^-1(Fw)，采用液体浅层培养的方法进行培养。2～3d后，发生第一次分裂，第10天，统计分裂频率为6l％，5周内形成大量的细胞团和小愈伤组织，统计植板率为1．1％，把小愈伤组织转到与原生质体培养基相同激素的MS固体培养基上增殖。当愈伤组织长到3～5mm大小时，接到分化培养基上，芽分化率为46.7％．分化出来的芽长到3～4cm长时，从基部切下，插入生根培养基，两星期左右即可长成完整植株。

Plant Regeneration from Hypocotyl Protoplasts of Brassica oleracea

The yield of purified protoplasts isolated from hypocotyl of Brassica olear-acea was 1. 5 X 106g -1 (FW)and they were cultured in a K8p liquid medium supplemented with 0. 2 mg/L 2, 4-D, 1mg/L NAA and 0. 5mg/L BA. The protoplasts started to divide after 2~3 days of culture, and the division frequency was 61% after 10 days. The cell clones and microcalli could be obtained in 5 weeks, then transferred to the proliferation MS medium supplemented with the same hormones as the K8p liquid medium. The plating frequency was about 1.1%. Calli of 3~5mm in diameter were transferred on MS differentiation medium, the frequency of shoot but formation was about 46. 7%. Whole plants were obtained upon transferring 3~4cm shoots to 1/2 MS medium with 0. 5mg/L IBA.