| 小鼠受精卵早期发育过程中PKB/Akt对p21蛋白表达及定位的影响 |
| |
| 引用本文： | 武迪迪,冯晨,刘莹,张杰,宗志宏,具英花,李雪松,于秉治.小鼠受精卵早期发育过程中PKB/Akt对p21蛋白表达及定位的影响[J].生物工程学报,2007,23(3):493-496. |
| |
| 作者姓名： | 武迪迪 冯晨 刘莹 张杰 宗志宏 具英花 李雪松 于秉治 |
| |
| 作者单位： | 中国医科大学基础医学院生物化学与分子生物学教研室,沈阳,110001 |
| |
| 基金项目： | 国家自然科学基金;国家重点基础研究发展计划(973计划);辽宁省博士科研项目 |
| |
| 摘 要： | 初步探讨在小鼠受精卵早期发育过程中PKB/Akt对p21蛋白表达及定位的影响。通过显微操作技术注射野生型、持续激活型及激酶失活型的PKB的mRNA,用免疫荧光方法检测p21蛋白的细胞定位、Western blot方法检测p21蛋白的表达。结果显示在注射不同形式的PKB mRNA后p21蛋白的表达无明显差别,但是细胞定位发生改变,PKB被激活后,p21蛋白滞留在胞浆中。因而初步认为在小鼠受精卵中,PKB/Akt通过影响p21的细胞定位而影响细胞周期的进程。
|
| 关 键 词： | 蛋白激酶B 细胞周期 小鼠受精卵 |
| 文章编号： | 1000-3061（2007）03-0493-04 |
| 修稿时间： | 2006-11-072006-12-14 |
The Effect of Protein Kinase B on the Expression and Location of p21 in Early Development of Mouse Fertilized Eggs |
| |
| WU Di-Di,FENG Chen,LIU Ying,ZHANG Jie,ZONG Zhi-Hong,JU Ying-Hu,LI Xue-Song and YU Bing-Zhi.The Effect of Protein Kinase B on the Expression and Location of p21 in Early Development of Mouse Fertilized Eggs[J].Chinese Journal of Biotechnology,2007,23(3):493-496. |
| |
| Authors: | WU Di-Di FENG Chen LIU Ying ZHANG Jie ZONG Zhi-Hong JU Ying-Hu LI Xue-Song and YU Bing-Zhi |
| |
| Institution: | Department of Biochemistry, School of Basic Medical Sciences, China Medical University, Shenyang 110001, China;Department of Biochemistry, School of Basic Medical Sciences, China Medical University, Shenyang 110001, China;Department of Biochemistry, School of Basic Medical Sciences, China Medical University, Shenyang 110001, China;Department of Biochemistry, School of Basic Medical Sciences, China Medical University, Shenyang 110001, China;Department of Biochemistry, School of Basic Medical Sciences, China Medical University, Shenyang 110001, China;Department of Biochemistry, School of Basic Medical Sciences, China Medical University, Shenyang 110001, China;Department of Biochemistry, School of Basic Medical Sciences, China Medical University, Shenyang 110001, China;Department of Biochemistry, School of Basic Medical Sciences, China Medical University, Shenyang 110001, China |
| |
| Abstract: | To investigate the effect of Protein kinase B on the expression and location of p21 in mouse early development. Immunopreciptation technology was used to detect the localization of p21 and Western blotting was used to analyze the expression of p21 after microinjecting mRNA of WT-PKB,myt-PKB and PKB-KD to mouse eggs. There was no obvious difference between the three kinds of mRNA in the expression of p21. But the cell localization altered. The p21 retain in cytoplasm after microjecting myt-PKB. In mouse fertilized egg PKB/Akt controls the cell cycle by changing the cell localization of p21. |
| |
| Keywords: | p21 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《生物工程学报》浏览原始摘要信息 |
| 点击此处可从《生物工程学报》下载免费的PDF全文 |
|
