| 小球藻病毒基因调控序列在大肠杆菌和真核藻中的调控活性研究 |
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| 引用本文： | 康明,韩继刚,刘平芳,叶寅,田波.小球藻病毒基因调控序列在大肠杆菌和真核藻中的调控活性研究[J].生物工程学报,2000,16(4):443-446. |
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| 作者姓名： | 康明 韩继刚 刘平芳 叶寅 田波 |
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| 作者单位： | 1. 中国科学院微生物研究所,北京,100080;河北大学生命科学学院,保定,071002
2. 中国科学院微生物研究所,北京,100080 |
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| 基金项目： | 海洋“863”项目(819-04-09-98)和国家自然科学基金项目(39870016)。 |
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| 摘 要： | 以小球藻病毒腺嘌呤甲基转移酶基因(amt)和主要外壳蛋白VP54基因的5′上游调控序列构建大肠杆菌和真核藻转化载体。以PRPL及CaMV35S启动子为阳性对照，研究了小球藻病毒来源的两种调控序列在E.coli和真核藻细胞中的启动活性。发现PAMT在4种E.coli菌株中都具有极强的调控活性，启动Luc基因表达而产生的酶活性高于PRPL 50～400倍；PVP54在DH5α中也具有较强的启动活性。同时PAMT在两种小球藻中启动GUS基因瞬时表达的能力也明显高于CaMV35S启动子，表明它们有可能在真核藻类遗传转化中具有很好的应用前景。
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| 关 键 词： | 病毒基因启动子，调控活性，E.coli，真核藻，瞬时表达 |
| 文章编号： | 1000-3061(2000)04-0443-04 |
| 修稿时间： | 1999-08-30 |
The Regulation Activity of Chlorella Virus Gene 5' Upstream Sequence in Escherichia coil and Eucaryotic Alage |
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| KANG Ming,HAN Ji-Gang,LIU Ping-Fang,YE Yin,TIEN Po.The Regulation Activity of Chlorella Virus Gene 5'''' Upstream Sequence in Escherichia coil and Eucaryotic Alage[J].Chinese Journal of Biotechnology,2000,16(4):443-446. |
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| Authors: | KANG Ming HAN Ji-Gang LIU Ping-Fang YE Yin TIEN Po |
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| Institution: | KANG Ming
,HAN Ji-Gang
;(Institute of Microbiology, The Chinese Academy of Sciences,Beijing, 100080;College of Life Science, Hebei Uniuersity, Baoding 071002);LIU Ping-Fang
,YE Yin
,TIEN Po
;(Institute of Microbiology, The Chinese Academy of Sciences,Beijing, 100080) |
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| Abstract: | The 5' upstream regions of adenine methyltransgerase gene and major coat protein gene (PAMT, PVP54) in Chlorella virus genomes were used to contract transformation vectors in E. coli and eukaryotic algae. The regulation activities of PAMT and PVP54 comparing with PRPL and CaMV35S promoters were analyzed in different E. coli strains and Chlorella species. It is found that the luciferase activity controlled by PAMT is 50-400 times higher than that controlled by PRPL. The regulation activity of PAMT in 2 Chlorella species is obviously higher than that of CaMV35S promoter. It is the first report that the 5' upstream region of Chlorella virus gene has strong regulation activity in eucaryotic algae. The result suggests this regulation sequence will have an excellent application in the eucaryotic algae genetic engineering. |
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| Keywords: | Virus gene promoter regulation activity E coli eucaryotic algae transient expression |
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