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| 山羊痘病毒TaqMan-MGB荧光定量PCR检测方法的建立与应用 | |
| 引用本文： | 程振涛,岳筠,李永明,许乐仁,王开功,周碧君,陈军义,李俊,江楠.山羊痘病毒TaqMan-MGB荧光定量PCR检测方法的建立与应用[J].生物工程学报,2009,25(3):464-472. |
| 作者姓名： | 程振涛 岳筠 李永明 许乐仁 王开功 周碧君 陈军义 李俊 江楠 |
| 作者单位： | 1. 贵州大学动物疫病研究所,贵阳,550025;贵州大学动物科学学院,贵阳,550025 2. 贵州大学动物科学学院,贵阳,550025 3. 贵阳医学院,贵阳,550025 |
| 基金项目： | 教育部科学技术研究重点项目(No.206132);;高层次人才科研条件特助经费项目(No.TZJF-200606)资助~~ |
| 摘 要： | 利用DNAStar分析了GenBank中所有8株羊痘病毒全基因序列,选取位于山羊痘病毒(AY077835)基因组gp064区域约64bp的基因片段,设计并合成了一对PCR引物和一条TaqMan-MGB探针,建立了FQ-PCR和标准曲线,并利用该方法对山羊痘临床皮肤痘疹材料和人工感染动物材料进行GPV核酸检测。结果表明,构建的FQ-PCR具有良好的敏感性、特异性、稳定性和临床应用性。该方法的建立为山羊痘临床快速高效地诊断和山羊痘病毒感染羊只的发生、发展及转归研究提供了一种有效的手段。 |
| 关 键 词： | 山羊痘病毒 TaqMan-MGB探针 荧光定量PCR 建立 应用 |
| 收稿时间： | 2008/10/27 0:00:00 |
Development and application of TaqMan-MGB real-time quantitative PCR assay for detection of goat pox virus |
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| Zhentao Cheng,Jun Yue,Yongming Li,Leren Xu,Kaigong Wang,Bijun Zhou,Junyi Chen,Jun Li and Nan Jiang.Development and application of TaqMan-MGB real-time quantitative PCR assay for detection of goat pox virus[J].Chinese Journal of Biotechnology,2009,25(3):464-472. | |
| Authors: | Zhentao Cheng Jun Yue Yongming Li Leren Xu Kaigong Wang Bijun Zhou Junyi Chen Jun Li and Nan Jiang |
| Institution: | Institute of Animal Disease, Guizhou University, Guiyang 550025, China; College of Animal Science, Guizhou University, Guiyang 550025, China;Institute of Animal Disease, Guizhou University, Guiyang 550025, China;Institute of Animal Disease, Guizhou University, Guiyang 550025, China; College of Animal Science, Guizhou University, Guiyang 550025, China;College of Animal Science, Guizhou University, Guiyang 550025, China;Institute of Animal Disease, Guizhou University, Guiyang 550025, China; College of Animal Science, Guizhou University, Guiyang 550025, China;Institute of Animal Disease, Guizhou University, Guiyang 550025, China; College of Animal Science, Guizhou University, Guiyang 550025, China;College of Animal Science, Guizhou University, Guiyang 550025, China;College of Animal Science, Guizhou University, Guiyang 550025, China;Guiyang Medical College, Guiyang 550025, China |
| Abstract: | The complete gene sequences of eight capripoxvirus strains in GenBank were aligned and analyzed with DNAStar software. We selected a size of 64 bp gene fragment that was located in gp064 region of goat pox virus (GPV) genome, and designed a pair of primers and a TaqMan-MGB probe against the gene fragment with Primer Express 2.0 software. Then, the fluorescence quantitative PCR (FQ-PCR) assay was developed and the standard curve of different dilution series was described. We extracted the DNA samples from cl... |
| Keywords: | goat pox virus TaqMan-MGB probe fluorescence quantitative PCR development application |
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