| 重组海洋细菌漆酶Lac15脱色人工合成纺织染料的潜力 |
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| 引用本文： | 房伟,方泽民,常飞,彭惠,张学成,肖亚中.重组海洋细菌漆酶Lac15脱色人工合成纺织染料的潜力[J].生物工程学报,2012,28(8):973-980. |
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| 作者姓名： | 房伟 方泽民 常飞 彭惠 张学成 肖亚中 |
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| 作者单位： | 安徽大学生命科学学院,安徽合肥230039;安徽省微生物与生物催化工程技术研究中心,安徽合肥230039 |
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| 基金项目： | 国家高技术研究发展计划 (863计划) (No. 2011AA09070305)，教育部留学回国人员科研项目，博士点专项基金 (No. 20093401110006)，安徽大学211工程创新团队计划项目经费资助。 |
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| 摘 要： | 以重组海洋细菌漆酶Lac15(rLac15)为生物催化剂,对蒽醌类和偶氮类染料进行脱色,考察了rLac15对人工合成纺织染料的脱色潜能。通过研究催化的介体、酶量、pH、染料浓度以及温度对脱色效率的影响,进一步优化了rLac15对部分蒽醌类和偶氮类人工合成纺织染料的脱色条件。以丁香酸甲酯为介体,在pH 8.5、45℃条件下反应1 h,20 U/L rLac15对100μmol/L偶氮染料类Acid Red 6B(AR-6B),Reactive Blue 194(M-2GE),Reactive Brilliant Orange(K-7R)和Reactive Blue 171(KE-R)具有较好的脱色效果,脱色率分别达到95%、93%、76%和61%。随着染料浓度的增加,脱色率呈下降趋势,但当染料浓度达到200μmol/L时,M-2GE和AR-6B仍可保持80%以上脱色率。在常温下,rLac15对AR-6B、M-2GE、K-7R和KE-R显示较高脱色率,25℃反应24 h,分别达到96%、86%、66%和66%。rLac15是具有常温以及偏碱性环境脱色能力的细菌漆酶,具有潜在工业应用价值。
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| 关 键 词： | 细菌漆酶 染料 脱色 介体 |
| 收稿时间： | 2012/1/11 0:00:00 |
Dye decolorization by bacterial laccase Lac15 |
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| Wei Fang,Zemin Fang,Fei Chang,Hui Peng,Xuecheng Zhang and Yazhong Xiao.Dye decolorization by bacterial laccase Lac15[J].Chinese Journal of Biotechnology,2012,28(8):973-980. |
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| Authors: | Wei Fang Zemin Fang Fei Chang Hui Peng Xuecheng Zhang and Yazhong Xiao |
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| Institution: | School of Life Science, Anhui University, Hefei 230039, Anhui, China; Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei 230039, Anhui, China;School of Life Science, Anhui University, Hefei 230039, Anhui, China; Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei 230039, Anhui, China;School of Life Science, Anhui University, Hefei 230039, Anhui, China; Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei 230039, Anhui, China;School of Life Science, Anhui University, Hefei 230039, Anhui, China; Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei 230039, Anhui, China;School of Life Science, Anhui University, Hefei 230039, Anhui, China; Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei 230039, Anhui, China;School of Life Science, Anhui University, Hefei 230039, Anhui, China; Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei 230039, Anhui, China |
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| Abstract: | |
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| Keywords: | bacterial laccase dye decolorization mediator |
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