| 乙酰羟酸合成酶同工酶基因ilvBN、ilvGM和ilvIH的表达及对除草剂抗性的比较 |
| |
| 引用本文： | 沈晶晶,李永丰,黄星,俞欣妍,何健,李顺鹏.乙酰羟酸合成酶同工酶基因ilvBN、ilvGM和ilvIH的表达及对除草剂抗性的比较[J].生物工程学报,2009,25(7):1007-1013. |
| |
| 作者姓名： | 沈晶晶 李永丰 黄星 俞欣妍 何健 李顺鹏 |
| |
| 作者单位： | 南京农业大学生命科学学院微生物学系农业部农业环境微生物工程重点开放实验室,南京,210095 |
| |
| 基金项目： | 国家自然科学基金项目(No.30500010)资助~~ |
| |
| 摘 要： | 乙酰羟酸合成酶(AHAS)是磺酰脲类和咪唑啉酮类等AHAS抑制剂类除草剂的作用靶标。获得抗此类除草剂的AHAS突变基因资源具有非常重要的理论和应用价值。本研究从抗甲磺隆菌株Klebsiella sp.HR11和甲磺隆敏感菌株Klebsiella pneumoniae MGH 78578中分别克隆到AHAS三种同工酶基因ilvBN、ilvGM和ilvIH。抗性菌株和敏感菌株AHAS三种同工酶基因在氨基酸水平上差异位点主要集中在ilvBN和ilvGM的大亚基上。将2株菌的ilvBN、ilvGM和ilvIH分别构建到表达载体pET29a(+)中,在Escherichia coli BL21(DE3)中进行表达,测得只有含菌株HR11 ilvBN和ilvGM的转化子细胞破碎液AHAS对各类AHAS抑制剂类除草剂具有较强的抗性,而含菌株HR11 ilvIH和菌株MGH78578 ilvBN、ilvGM和ilvIH的转化子细胞破碎液AHAS对各类AHAS抑制剂类除草剂敏感。
|
| 关 键 词： | AHAS抑制剂类除草剂 乙酰羟酸合酶同工酶 ilvBN ilvGM ilvIH 除草剂抗性 |
| 收稿时间： | 2/5/2009 12:00:00 AM |
Expression of acetohydroxyacid synthase isozyme genes ilvBN, ilvGM, ilvIH and their resistance to AHAS-inhibitor herbicides |
| |
| Jingjing Shen,Yongfeng Li,Xing Huang,Xinyan Yu,Jian He and Shunpeng Li.Expression of acetohydroxyacid synthase isozyme genes ilvBN, ilvGM, ilvIH and their resistance to AHAS-inhibitor herbicides[J].Chinese Journal of Biotechnology,2009,25(7):1007-1013. |
| |
| Authors: | Jingjing Shen Yongfeng Li Xing Huang Xinyan Yu Jian He and Shunpeng Li |
| |
| Institution: | Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China;Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China;Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China;Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China;Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China;Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China |
| |
| Abstract: | Acetohydroxyacid synthase (AHAS) catalyses the first reaction in the pathway for synthesis of the branched-chain amino acids. AHAS is the target for sulfonylurea, imidazolinone and other AHAS-inhibitor herbicides. Herbicides-resistant AHAS genes have potential application in plant transgenetic engineering and development of new generation herbicide. The AHAS isozyme genes ilvBN, ilvGM and ilvIH were cloned from metsulfuron-methyl resistant strain Klebsiella sp. HR11 and metsulfuron-methyl sensitive strain K... |
| |
| Keywords: | AHAS-inhibitor herbicides AHAS isozymes ilvBN ilvGM ilvIH herbicides-resistance |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《生物工程学报》浏览原始摘要信息 |
| 点击此处可从《生物工程学报》下载免费的PDF全文 |
|
