| 微生物法生产丙烯酰胺的研究（Ⅰ）—腈水合酶产生菌株的培养和高活力的表达 |
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| 引用本文： | 陈跖,赵建勋,等.微生物法生产丙烯酰胺的研究（Ⅰ）—腈水合酶产生菌株的培养和高活力的表达[J].生物工程学报,2002,18(1):55-58. |
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| 作者姓名： | 陈跖 赵建勋 |
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| 作者单位： | [1]清华大学化工系生物化工研究所，北京100094 [2]山东胜利石油管理局，东营257019 |
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| 摘 要： | 研究了丙烯酰胺生产菌株的培养条件。通过对培养过程pH值调控、培养基补料以及诱导剂加入量的研究，使发酵液的腈水合酶的活力达到了6567u/mL菌液。这一酶活是国内外所见报道中最高的。进一步进行了丙烯腈的酶催化水合实验，产物中并没有发现副产物丙烯酸，说明在提高腈水合酶的同时，酰胺酶的活力并没有明显体现这一试验结果为工厂化生产改造以及新工艺的研究打下了基础。
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| 关 键 词： | 微生物法 丙烯酰胺 腈水合酶产生菌株 培养 高活力 表达 |
| 文章编号： | 1000-3061(2002)01-0055-04 |
| 修稿时间： | 2001年6月20日 |
Study on production of acrylamide by microbial method (I)--Culture of bacterium cells and expression of high activity of nitrile hydratase] |
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| CHEN Zhi\ SUN Xu-Dong\ SHI Yue\ SHEN Zhong-Yao.Study on production of acrylamide by microbial method (I)--Culture of bacterium cells and expression of high activity of nitrile hydratase][J].Chinese Journal of Biotechnology,2002,18(1):55-58. |
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| Authors: | CHEN Zhi\ SUN Xu-Dong\ SHI Yue\ SHEN Zhong-Yao |
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| Institution: | Tsinghua University, Department of Chemical Engineering, Institute of Biochemical Engineering, Beijing 100094, China. |
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| Abstract: | The cultural conditions for the growth of Norcardia cell were studied in this paper.Controlling pH value,adding nutrient and optimizing the quantity of inducer during cultivation,the activity of nitrile hydratase reached 6567u/mL (culture medium),which was the highest value appeared in native journals.In the farther hydratase experiments,no by-product,crylic acid,was detected.It showed that the activity of amidase was not promoted obviously while the activity of nitrile hydratase was increased greatly.The results set a strong foundation for the industrial application and the research on new technology. |
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| Keywords: | acrylamide nitrile hydratase a midase |
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