| 新一代高产甘油重组菌的构建及其初步发酵 |
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| 引用本文： | 刘爱玲,饶志明,马正,诸葛斌,方慧英,诸葛健.新一代高产甘油重组菌的构建及其初步发酵[J].生物工程学报,2009,25(6):946-952. |
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| 作者姓名： | 刘爱玲 饶志明 马正 诸葛斌 方慧英 诸葛健 |
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| 作者单位： | 江南大学工业生物技术教育部重点实验室丁业微生物研究中心,无锡,214122 |
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| 基金项目： | 国家自然科学基金项目(No.20676053);;国家高技术研究发展计划(863计划)(No.2006AA020103);;江苏省青年科技创新人才(学术带头人)基金(No.BK2006504);;长江学者和创新团队发展计划(No.IRT0532)资助~~ |
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| 摘 要： | 产甘油假丝酵母(Candida glycerinogenes WL2002-5)是一株发酵生产甘油的工业化菌株。为进一步提高其产甘油能力,本研究利用前期研究中成功克隆的产甘油假丝酵母中甘油合成关键酶3-磷酸甘油脱氢酶基因CgGPD1,构建根癌农杆菌双元载体pCAM3300-zeocin-CgGPD1后,电击转化根癌农杆菌LBA4404,通过根癌农杆菌介导法(ATMT)转化产甘油假丝酵母,构建了产甘油假丝酵母重组菌。并从中筛选出一株酶活力和产甘油性能较好的产甘油假丝酵母重组菌株C.g-G8。以葡萄糖为底物摇瓶发酵96h后,重组菌C.g-G8的甘油产量比野生型菌株Candida glycerinogene提高18.06%,平均耗糖速率提高12.97%,平均酶活力提高27.55%。本研究成功利用ATMT法转化产甘油假丝酵母构建新一代高产甘油菌株。
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| 关 键 词： | 产甘油假丝酵母 胞浆-3-磷酸甘油脱氢酶 根癌农杆菌介导转化 |
| 收稿时间： | 2009/1/10 0:00:00 |
Construction and fermentation of a recombinant Candida glycerinogenes strain with high glycerol production |
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| Ailing Liu,Zhiming Rao,Zheng M,Bin Zhuge,Huiying Fang and Jian Zhuge.Construction and fermentation of a recombinant Candida glycerinogenes strain with high glycerol production[J].Chinese Journal of Biotechnology,2009,25(6):946-952. |
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| Authors: | Ailing Liu Zhiming Rao Zheng M Bin Zhuge Huiying Fang and Jian Zhuge |
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| Institution: | The Key Laboratory of Industrial Biotechnology of Ministry of Education, Research Center of Industrial Microbiology, School of Biotechnology, Jiangnan University, Wuxi 214122, China;The Key Laboratory of Industrial Biotechnology of Ministry of Education, Research Center of Industrial Microbiology, School of Biotechnology, Jiangnan University, Wuxi 214122, China;The Key Laboratory of Industrial Biotechnology of Ministry of Education, Research Center of Industrial Microbiology, School of Biotechnology, Jiangnan University, Wuxi 214122, China;The Key Laboratory of Industrial Biotechnology of Ministry of Education, Research Center of Industrial Microbiology, School of Biotechnology, Jiangnan University, Wuxi 214122, China;The Key Laboratory of Industrial Biotechnology of Ministry of Education, Research Center of Industrial Microbiology, School of Biotechnology, Jiangnan University, Wuxi 214122, China;The Key Laboratory of Industrial Biotechnology of Ministry of Education, Research Center of Industrial Microbiology, School of Biotechnology, Jiangnan University, Wuxi 214122, China |
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| Abstract: | Candida glycerinogenes WL2002-5(C.g) is an important industrial strain for glycerol production.To further improve glycerol production,we reconstructed a binary vector pCAM3300-zeocin-CgGPD1,introduced it to Agrobacterium tumefaciens LBA4404 by electroporation,and then transformed the T-DNA harboring the CgGPD1 to Candida glycerinogenes by Agrobacterium tumefaciens-mediated transformation(ATMT).After 96 h fermentation with glucose as the substrate,we screened a transformant named C.g-G8 with high glycerol pr... |
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| Keywords: | Candida glycerinogenes glycerol-3-phosphate dehydrogenase Agrobacterium tumefaciens-mediated transformation |
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