| 电镜原位分子杂交技术及其应用 |
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| 引用本文： | 谢潮添,杨盛昌,缪颖,陈德海,张平.电镜原位分子杂交技术及其应用[J].生物工程学报,2002,18(2):249-252. |
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| 作者姓名： | 谢潮添 杨盛昌 缪颖 陈德海 张平 |
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| 作者单位： | 厦门大学生命科学院植物基因与基因技术研究所,厦门,361005 |
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| 基金项目： | 国家自然科学基金资助 (No .39870 46 1)~~ |
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| 摘 要： | 原位分子杂交技术应用于电镜水平，观察标记的特定DNA、mRNA和RHA在细胞和/或组织内的超微结构定位的方法，称为电镜原位分子杂交技术。本文综述了电镜原位分子杂交技术的建立及其分类，并详细介绍了非放射性电镜原位分子杂交技术的基本操作程序及注意事项，最后对电镜原位分子杂交技术的应用做了简要介绍。
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| 关 键 词： | 电镜，原位分子杂交技术，操作程序 |
| 文章编号： | 1000-3061(2002)02-0249-04 |
| 修稿时间： | 2001年9月18日 |
The Electron Microscopic in situ Hybridization and Its Application |
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| XIE Chao,Tian\\,YANG Sheng,Chang\,MIAO Ying\,CHEN De,Hai\,ZHANG Ping.The Electron Microscopic in situ Hybridization and Its Application[J].Chinese Journal of Biotechnology,2002,18(2):249-252. |
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| Authors: | XIE Chao Tian\\ YANG Sheng Chang\ MIAO Ying\ CHEN De Hai\ ZHANG Ping |
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| Institution: | Institution of Plant Gene and Gene Technology, School of Life Sciences, Xiamen University, Xiamen 361005, China. ctxie@163.net |
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| Abstract: | The technique of electron microsco-pic in situ hybridization is applying in situ hybridization at the electron microscopic level. It is mainly used in the ultrastructural localization of the lablled DNA, RNA and RHA in a cell and/or a tissue. In this paper I mainly elaborated its establishment and classification, and the operation procedure of nonradioactive electron microscopic in situ hybridization and some points for attention. In the end I also discussed its application for research. |
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| Keywords: | electron microscope \%in situ\% hybridization protocol |
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