| 慢性高剂量胰岛素刺激猪脂肪细胞脂肪分解 |
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| 引用本文： | 杨永青,鞠大鹏,张明涛,杨公社.慢性高剂量胰岛素刺激猪脂肪细胞脂肪分解[J].生物工程学报,2009,25(1):0016-0022. |
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| 作者姓名： | 杨永青 鞠大鹏 张明涛 杨公社 |
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| 作者单位： | 1. 西北农林科技大学,动物脂肪沉积与肌肉发育实验室,杨凌,712100;山西师范大学生命科学学院,临汾,041000
2. 西北农林科技大学,动物脂肪沉积与肌肉发育实验室,杨凌,712100 |
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| 基金项目： | 国家高技术研究发展计划(“863计划”) (No. 2006AA10Z138)资助。 |
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| 摘 要： | 为研究慢性高剂量胰岛素对猪脂肪细胞脂肪分解的影响及其分子机制, 分化的猪脂肪细胞在PKA(Protein kinase A)或ERK(Extracellular signal-related kinase)抑制剂预处理或不处理的情况下, 再用不同浓度的胰岛素(0、200、400、800、1600 nmol/L)处理不同时间(24、48、72、96 h), 通过测定甘油释放量检测脂肪细胞的脂解率; 采用RT-PCR和Western blotting检测perilipin A和PPARg2的mRNA和蛋白表达。结果显示, 慢性高剂量胰岛素以剂量和时间依赖性的方式刺激猪脂肪细胞的脂肪分解, 并削弱脂肪细胞对异丙肾上腺素刺激的脂解应答; 同时显著下调perilipin A和PPARg2的mRNA及蛋白表达; 另外, PKA和ERK抑制剂均显著抑制胰岛素刺激的脂肪分解, 但仅ERK抑制剂显著逆转perilipin A基因表达的下调。由此推测, 慢性高剂量胰岛素通过ERK通路抑制perilipin A的表达, 进而刺激猪脂肪细胞的脂肪分解。
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| 关 键 词： | 胰岛素 猪脂肪细胞 脂肪分解 |
| 收稿时间： | 2008/6/17 0:00:00 |
Chronic high dose of insulin stimulates lipolysis in porcine adipocytes |
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| Yongqing Yang,Dapeng Ju,Mingtao Zhang and Gongshe Yang.Chronic high dose of insulin stimulates lipolysis in porcine adipocytes[J].Chinese Journal of Biotechnology,2009,25(1):0016-0022. |
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| Authors: | Yongqing Yang Dapeng Ju Mingtao Zhang and Gongshe Yang |
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| Institution: | Laboratory of Animal Fat Deposition and Muscle Development, Northwest A & F University, Yangling 712100, China; College of Life Science, Shanxi Normal University, Linfen 041000, China;Laboratory of Animal Fat Deposition and Muscle Development, Northwest A & F University, Yangling 712100, China;Laboratory of Animal Fat Deposition and Muscle Development, Northwest A & F University, Yangling 712100, China;Laboratory of Animal Fat Deposition and Muscle Development, Northwest A & F University, Yangling 712100, China |
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| Abstract: | To explore the effect of chronic high dose of insulin on lipolysis in porcine adipocytes and the underlying molecular regulation mechanisms,we cultured primary porcine adipocytes and incubated them with different concentrations of insulin(0,200,400,800,1600 nmol/L) for 24?96 h in the absence or presence of specific protein kinase A(PKA) inhibitor or extracellular signal-related kinase(ERK) inhibitor.Then,we measured the glycerol release into the culture media as an indicator of the lipolysis,and observed th... |
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| Keywords: | perilipin A |
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