| 13C代谢流量分析发展30年 |
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| 引用本文： | 姚瑞莲.13C代谢流量分析发展30年[J].生物工程学报,2021,37(5):1510-1525. |
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| 作者姓名： | 姚瑞莲 |
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| 作者单位： | 上海交通大学 生命科学技术学院 微生物代谢国家重点实验室，上海 200240 |
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| 基金项目： | 国家重点研发计划 (No. 2019YFA0904304) 资助。 |
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| 摘 要： | ~(13)C代谢流量分析(~(13)C metabolic flux analysis,~(13)C-MFA),是通过标记实验分析蛋白氨基酸或胞内代谢物同位素标记异构体的分布情况,从而准确定量胞内反应速率。该技术在系统理解细胞代谢特性、指导代谢工程改造和揭示病理生理学等方面起着重要作用,引起研究者的广泛重视。文中重点综述了代谢流分析30年的发展历程,尤其在工业生物技术和生物医药领域的应用,并对未来的发展方向进行展望。
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| 关 键 词： | 13C代谢流量分析，代谢工程，工业生物技术，生物医药 |
| 收稿时间： | 2020/11/23 0:00:00 |
Thirty years development of 13C metabolic flux analysis: a review |
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| Ruilian Yao.Thirty years development of 13C metabolic flux analysis: a review[J].Chinese Journal of Biotechnology,2021,37(5):1510-1525. |
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| Authors: | Ruilian Yao |
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| Institution: | State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China |
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| Abstract: | 13C metabolic flux analysis (13C-MFA) enables the precise quantification of intracellular metabolic reaction rates by analyzing the distribution of mass isotopomers of proteinogenic amino acids or intracellular metabolites through 13C labeling experiments. 13C-MFA has received much attention as it can help systematically understand cellular metabolic characteristics, guide metabolic engineering design and gain mechanistic insights into pathophysiology. This article reviews the advances of 13C-MFA in the past 30 years and discusses its potential and future perspective, with a focus on its application in industrial biotechnology and biomedicine. |
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| Keywords: | 13C metabolic flux analysis metabolic engineering industrial biotechnology biomedicine |
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