| 蛙皮素-蜂毒素杂合肽基因在大肠杆菌中的克隆与诱导表达 |
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| 引用本文： | 黄音,刘飞鹏,周天鸿,朱嘉明.蛙皮素-蜂毒素杂合肽基因在大肠杆菌中的克隆与诱导表达[J].生物工程学报,2001,17(2):207-210. |
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| 作者姓名： | 黄音 刘飞鹏 周天鸿 朱嘉明 |
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| 作者单位： | [1]上海复旦大学遗传所科学楼618 [2]暨南大学生物工程系，广州510632 |
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| 基金项目： | 广东省自然科学基金资助(970636). |
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| 摘 要： | 抗菌肽 (Ａｎｔｉｂａｃｔｅｒｉａｌｐｅｐｔｉｄｅｓ)原指昆虫体内经诱导产生的一类分子量在 4ｋＤ左右 ,具有抗菌活性的碱性多肽物质2 ] 。这类抗菌剂最初是从昆虫、哺乳动物、两栖动物等的防御系统中分离得到的。抗菌肽对革兰氏阳性及阴性细菌、病毒、原虫和发生病变的真核细胞等有杀伤作用 ,具有很强的广谱抑菌作用 ,但对正常哺乳动物细胞无杀伤作用3 ] 。蛙皮素还具有抗肿瘤活性而没有溶血活性4 ] ,虽然蜂毒素具有溶血活性 ,但其溶血功能区位于Ｃ端的亲水区域5] ,两者的极性区域正好相反。据研究表明抗菌肽都会形成两亲螺旋结构的…
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| 关 键 词： | 抗菌肽 MA－E基因 大肠杆菌 抗菌活性 表达 克隆 蛙皮素－蜂毒素杂合肽基因 |
| 文章编号： | 1000-3061(2001)02-0207-04 |
| 修稿时间： | 2000年7月7日 |
Cloning and Expression of a Synthetic Gene Encoding Magainin-Melittin Hybrid Peptide in Escherichia coli and Studies on its Antibacterial Activity |
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| HUANG Yin\ LIU Fei\|Peng\ \ ZHOU Tian\|Hong\ ZHU Jia\|Ming.Cloning and Expression of a Synthetic Gene Encoding Magainin-Melittin Hybrid Peptide in Escherichia coli and Studies on its Antibacterial Activity[J].Chinese Journal of Biotechnology,2001,17(2):207-210. |
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| Authors: | HUANG Yin\ LIU Fei\|Peng\ \ ZHOU Tian\|Hong\ ZHU Jia\|Ming |
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| Institution: | Department of Biotechnology, Jinan University, Guangzhou 510632, China. |
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| Abstract: | A hybrid peptide gene was designed and synthesized. Its encoding peptide is constructed from residues 3-14 of magainin and residues 1-13 of melittin. The MA-E gene was cloned into plasmids pUC18 and pBV220. By DNA sequencing, the whole sequences of this gene is confirmed to be correct. The recombinant plasmid pBMA-E was expressed in E. coli DH5 alpha. A gene product band can be seen with Tricine-SDS-PAGE. The MA-E hybrid peptide was purified by immobilized metal affinity chromatography. Bioactivity assay was carried out in liquid turbidity method. The bactericide value to E. coli K12D31 is 0.182. |
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| Keywords: | magainin melittin hybrid peptide gene expression |
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