|
|||||
|
|
| H3N2亚型猪流感病毒M1基因克隆及表达特性分析 | |
| 引用本文： | 郭林,王晓杜,刘庆伟,沈阳,邱亚峰,李向东,罗满林,马志永.H3N2亚型猪流感病毒M1基因克隆及表达特性分析[J].生物工程学报,2009,25(5):672-678. |
| 作者姓名： | 郭林 王晓杜 刘庆伟 沈阳 邱亚峰 李向东 罗满林 马志永 |
| 作者单位： | 1. 中国农业科学院上海兽医研究所兽医公共卫生研究室,上海,200241;华南农业大学兽医学院,广州,510642 2. 中国农业科学院上海兽医研究所兽医公共卫生研究室,上海,200241 3. 华南农业大学兽医学院,广州,510642 |
| 基金项目： | 上海市浦江人才计划(No.07pj14109);;中央级公益性科研院所基本科研业务费专项资金重点项目(No.64)资助~~ |
| 摘 要： | 从H3N2亚型猪流感病毒感染的鸡胚尿囊液中提取病毒基因组RNA,采用RT-PCR方法克隆M1全长基因,将M1基因亚克隆至pET-28a(+)表达载体中,构建重组表达质粒pET-28a-M1,将该重组质粒转化大肠杆菌BL21并经IPTG诱导表达。诱导产物经SDS-PAGE电泳分析显示重组蛋白M1获得大量表达,表达蛋白纯化后免疫Wistar大鼠制备多克隆抗体。Westernblotting检测结果表明制备的抗M1蛋白多克隆抗体可以识别大肠杆菌表达的M1蛋白和病毒感染细胞的病毒M1蛋白。构建M1基因真核重组质粒p3xFLAG-CMV-7.1-M1并转染Vero细胞,Western blotting检测表明抗M1蛋白多克隆抗体可以识别在Vero细胞中得到表达的M1蛋白,成功建立M1基因的真核表达系统。分析了病毒感染过程中M1蛋白的变化及其作为病毒复制指示分子的可能性。鼠源M1蛋白多克隆抗体的制备和M1基因真核重组表达质粒的构建,为进一步研究猪流感病毒的复制机理以及M1蛋白在病毒复制过程中的生物学功能奠定了基础。 |
| 关 键 词： | 猪流感病毒 M1基因 克隆 基因表达 |
| 收稿时间： | 2008/11/11 0:00:00 |
Cloning and characterization of M1 gene of H3N2 subtype swine influenza virus |
|
| Lin Guo,Xiaodu Wang,Qingwei Liu,Yang Shen,Yafeng Qiu,Xiangdong Li,Manlin Luo and Zhiyong Ma.Cloning and characterization of M1 gene of H3N2 subtype swine influenza virus[J].Chinese Journal of Biotechnology,2009,25(5):672-678. | |
| Authors: | Lin Guo Xiaodu Wang Qingwei Liu Yang Shen Yafeng Qiu Xiangdong Li Manlin Luo and Zhiyong Ma |
| Institution: | Department of Veterinary Public Health, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China; College of Veterinary Medicine, South China Agricultural University, Guangzhou 510624, China;Department of Veterinary Public Health, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China;Department of Veterinary Public Health, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China;Department of Veterinary Public Health, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China;Department of Veterinary Public Health, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China;Department of Veterinary Public Health, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China;College of Veterinary Medicine, South China Agricultural University, Guangzhou 510624, China;Department of Veterinary Public Health, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China |
| Abstract: | |
| Keywords: | swine influenza virus M1 gene cloning gene expression |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《生物工程学报》浏览原始摘要信息 | |
| 点击此处可从《生物工程学报》下载免费的PDF全文 | |