| 雌激素受体关联受体α 调节脂肪细胞甘油三酯分解 |
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| 引用本文： | 鞠大鹏,何晶晶,郑雪莉,赵丽丽,杨公社.雌激素受体关联受体α 调节脂肪细胞甘油三酯分解[J].生物工程学报,2011,27(1):18-25. |
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| 作者姓名： | 鞠大鹏 何晶晶 郑雪莉 赵丽丽 杨公社 |
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| 作者单位： | 西北农林科技大学动物科技学院,动物脂肪沉积与肌肉发育实验室,杨凌,712100 |
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| 基金项目： | 国家高技术研究发展计划 (863计划) (No. 2006AA10Z138) 资助。 |
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| 摘 要： | 雌激素受体关联受体a (Estrogen-related receptor a，ERRα) 是调控机体能量代谢的关键转录调控因子，也是脂肪生成的关键调控者。为研究ERRα对脂肪细胞甘油三酯分解的影响及其分子机制，分化的猪脂肪细胞在PKA (Protein kinase A) 或/和ERK (Extracellular signal-related kinase) 抑制剂预处理和不处理的情况下，再用Ad-ERRα侵染或XCT790处理48 h。通过测定脂肪细胞中甘油三酯浓度和培养液中的甘油释放量分析脂肪细胞的脂解变化；Western blotting方法检测PPARγ (Peroxisome proliferator-activated receptor γ，PPARγ)、perilipin A、p-perilipin A、HSL (Hormone sensitive lipase，HSL) 和ATGL (Adipose triglyceride lipase，ATGL) 蛋白表达。结果显示，ERRα显著促进猪脂肪细胞分化及甘油三酯积累，同时促进了甘油三酯水解；分别及同时阻断PKA和ERK通路并不影响ERRα对脂肪细胞甘油释放的促进作用；ERRα显著上调HSL、ATGL、PPARγ及perilipin A蛋白表达，但p-perilipin A水平并未发生变化。推测过量表达ERRα可能导致HSL和ATGL蛋白表达上调并促进甘油三酯水解，从而为脂肪细胞分化提供更多的游离脂肪酸 (Free fat acid，FFA) 作为甘油三酯合成周转的底物。
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| 关 键 词： | 猪，ERRα，甘油三酯水解，脂肪细胞 |
| 收稿时间： | 2010/3/24 0:00:00 |
| 修稿时间： | 2010/5/17 0:00:00 |
Role of estrogen-related receptor alpha in adipocyes lipolysis |
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| Dapeng Ju,Jingjing He,Xueli Zheng,Lili Zhao and Gongshe Yang.Role of estrogen-related receptor alpha in adipocyes lipolysis[J].Chinese Journal of Biotechnology,2011,27(1):18-25. |
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| Authors: | Dapeng Ju Jingjing He Xueli Zheng Lili Zhao and Gongshe Yang |
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| Institution: | Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China;Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China;Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China;Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China;Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China |
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| Abstract: | Estrogen-related receptor a (ERRalpha) is a key regulator for energy metabolism and adipogenesis. However, its role in lipolysis is unknown. To study the function of ERRalpha in lipolysis, primary cultured differentiated porcine adipocytes were treated by a specific inverse agonist XCT790 or infected with adenoviral vector expressed ERRalpha for 48 h, in the absence and/or presence of specific protein kinase A (PKA) inhibitor or extracellular signal-related kinase (ERK) inhibitor. Then, we measured the triglyceride (TG) content and the glycerol release into the culture media to analysis the effect of ERRalpha on lipolysis; Further, we analyzed the expression of PPARgamma, perilipin A, p-perilipin A, HSL and ATGL with Western blotting. Here, we found that ERRalpha significantly increased adipocytes differentiation, TG accumulation and glycerol release. Separately or simultaneously block the PKA and ERK pathway do not significantly altered the effect of ERRalpha on glycerol release. ERRalpha significantly up-regulated the proteins expression of PPARgamma, perilipin A, HSL and ATGL, while the p-perilipin A protein level was not significantly changed. These findings imply that ERRalpha could increase lipolysis via up-regulating HSL and ATGL, thereby to supply more FFA as substrate for a larger turnover of cellular triglyceride pool during adipocytes differentiation. |
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