| 利用定点突变分析白念珠菌依赖铁基因FRP1启动子元件 |
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| 引用本文： | 桂磊,梁勇,魏东盛,郑雯,邢来君,李明春.利用定点突变分析白念珠菌依赖铁基因FRP1启动子元件[J].生物工程学报,2008,24(8):1348-1353. |
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| 作者姓名： | 桂磊 梁勇 魏东盛 郑雯 邢来君 李明春 |
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| 作者单位： | 南开大学微生物学系,分子微生物学与技术教育部重点实验室,天津,300071 |
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| 基金项目： | 国家自然科学基金(No. 30570096), 教育部博士点基金资助项目(No. 20070055011), 教育部留学回国人员科研启动基金(教外司留(2005)546号)资助。 |
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| 摘 要： | 通过对白念珠菌高铁还原酶基因FRP1启动子进行突变分析, 确认启动子中特殊调控元件。我们通过分析FRP1起始密码子上游1000 bp序列发现在-160 和-650处有2个推测的Rim101p结合位点, 对其分别进行定点突变, 然后构建启动子与报告基因LacZ融合质粒, 转化整合到白念珠菌rim101-/-株和野生株中, 检测不同缺铁条件下b-半乳糖苷酶活性。结果发现碱性条件, Rim101p能够正向调控FRP1的表达; 启动子-160处突变对启动子功能影响较弱, 而-650突变使启动子活性大大降低, 此结果和双突变的结果相同, 表明Rim101p主要通过与启动子-650处结合位点相互作用来调控FRP1的表达。
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| 关 键 词： | 定点突变 白念珠菌 Rim101蛋白 高铁还原酶基因 |
| 收稿时间： | 2007/12/7 0:00:00 |
Regulating Promoter Element of Iron-dependent Gene FRP1 in Candida albicans by Site-directed Mutation |
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| Lei Gui,Yong Liang,Dongsheng Wei,Wen Zheng,Laijun Xing and Mingchun Li.Regulating Promoter Element of Iron-dependent Gene FRP1 in Candida albicans by Site-directed Mutation[J].Chinese Journal of Biotechnology,2008,24(8):1348-1353. |
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| Authors: | Lei Gui Yong Liang Dongsheng Wei Wen Zheng Laijun Xing and Mingchun Li |
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| Institution: | Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, Nankai University, Tianjin 300071, China;Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, Nankai University, Tianjin 300071, China;Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, Nankai University, Tianjin 300071, China;Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, Nankai University, Tianjin 300071, China;Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, Nankai University, Tianjin 300071, China;Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, Nankai University, Tianjin 300071, China |
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| Abstract: | |
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| Keywords: | site-directed mutation Candida albicans Rim101 protein ferric reductase gene |
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