动物乳腺生物反应器的现状和趋势

利用转基因家畜的乳腺生产人类重组蛋白，可以高效获得安全、足量的药用蛋白。本文针对乳腺生物反应器的成功研制，从目的基因的选择、载体构建、转基因技术等方面探讨了动物乳腺生物反应器的研究现状。分析了提高转基因效率和外源蛋白表达水平的技术途径，提出了降低总体成本的战略措施。特别探讨了利用Cre-loxP系统发展“体细胞打靶体细胞核移植技术体系”，高效生产乳腺生物反应器动物的可能性。

Transgenic Animals Bioreactors

The production of human recombinant proteins in milk of transgenic farm animals offers a safe, very cost effective source of commercially important proteins that cannot be produced as efficiently in adequate quantities by other methods. This review has summarized the current status of gene selection, vector construct, transgenic methods, economics, and obvious potential in transgenic animals bioreactors. Recently, a more powerful approach was adopted in the transgenic animals founded on the application of nuclear transfer. As we will illustrate, this strategy presents a breakthrough in the overall efficiency of generating transgenic farm animals, product consistency, and time of product development. The successful adaptation of Cre / lox P mediated site specific DNA recombination systems in farm animals will offer unprecedented possibilities for generating transgenic animals.