水稻转座子突变体库的构建及突变类型的遗传分析

利用根癌农杆菌介导的遗传转化法，把玉米转座子Ac片段(合成转座酶)和Ds因子(转座序列)分别导入粳稻品种中花11，获得了400多个转化株系。有63个株系的转基因T₀代植株或T₁代植株出现了可见的形态变异，包括抗病、白苗、黄苗、白条斑叶、有色秆、矮秆、匍匐茎、雄性不育、抽穗早、抽穗迟、多倍体等突变类型。对突变性状的形态及分子生物学分析，有些突变类型可能与转座子插入有关。

Construction of Transgenic Rice Populations by Inserting the Maize Transponson Ac/Ds and Genetic Analysis for Several Mutants

An efficient and rapid gene transformation system of rice mediated by Agrobacterium tumefaciens was used. Calli induced from immature and mature embryos of Zhonghua No. 11, a japonic rice variety, were cultured with the A. tumefaciens strain EHA105 harboring the superbinary plasmid pDsBar1300 or pUBITs separately, and more than 400 independent transgenic lines inserted Ds element or Ac fragment were obtained. Some visible mutants in T0 or T1 generation were found, consisting of disease resistance, albino, dwarf, male sterile, chlorosis, early heading, late heading, stripe, etc. From the phenotype analysis, a few mutants such as dwarf and male sterile seemed to be linked to the Basta resistance and the transposon.