| 以生物合成为基础的红霉素A的产量提高和结构改造 |
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| 引用本文： | 陈单丹,吴杰群,刘文.以生物合成为基础的红霉素A的产量提高和结构改造[J].生物工程学报,2015,31(6):939-954. |
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| 作者姓名： | 陈单丹 吴杰群 刘文 |
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| 作者单位： | 中国科学院上海有机化学研究所 湖州生物制造创新中心，浙江 湖州 313000,中国科学院上海有机化学研究所 湖州生物制造创新中心，浙江 湖州 313000,中国科学院上海有机化学研究所 湖州生物制造创新中心，浙江 湖州 313000 |
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| 基金项目： | 国家自然科学基金 (Nos. 81302674, 31430005, 91213303, 91413101) 资助。 |
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| 摘 要： | 红霉素A是一种广谱大环内酯类抗生素,在临床上应用广泛。其生物合成包括由聚酮合酶催化的十四元环骨架形成,以及羟基化、糖基化、甲基化后修饰。基于对红霉素A生物合成机制的认识,可以对产生菌种进行定向的遗传操作,达到产量提高和结构改造等目的。本文综述了近年来在红霉素A高产菌株改造和化学结构衍生方面所取得的研究进展,为相关研究人员提供参考。
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| 关 键 词： | 红霉素，生物合成，产量提高，结构改造 |
| 收稿时间： | 2014/12/25 0:00:00 |
Biosynthesis-based production improvement and structure modification of erythromycin A |
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| Dandan Chen,Jiequn Wu and Wen Liu.Biosynthesis-based production improvement and structure modification of erythromycin A[J].Chinese Journal of Biotechnology,2015,31(6):939-954. |
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| Authors: | Dandan Chen Jiequn Wu and Wen Liu |
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| Institution: | Huzhou Center of Bio-synthetic Innovation, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Huzhou 313000, Zhejiang, China,Huzhou Center of Bio-synthetic Innovation, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Huzhou 313000, Zhejiang, China and Huzhou Center of Bio-synthetic Innovation, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Huzhou 313000, Zhejiang, China |
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| Abstract: | Erythromycin A is a clinically important macrolide antibiotic with broad-spectrum activity. Its biosynthesis involves the formation of the 14-membered skeleton catalyzed by polyketide synthases, and the modification steps such as hydroxylation, glycosylation and methylation. Based on the understanding of the biosynthetic mechanism, it is reliable to genetically manipulate the erythromycin A-producing strain for production improvement and structure modification. In this paper, we reviewed the progress regarding erythromycin A in high-producing strain construction and chemical structure derivation, to provide insights for further development. |
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| Keywords: | erythromycin biosynthesis production improvement structure modification |
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