烟草花叶病毒（普通株中国分离物）及其弱毒苗—N14（番茄株）组织 …

用双脱氧未端经终止法对侵染性烟草共现毒普通株中国分离物（ＴＭＶ－ｖｉｒｌｇａｒ，Ｃｈｉｎｅｓｅ ｌｓｏｂｌａｔｅ，ＴＭＶ－Ｃｖ）和番茄株弱毒轩ＴＭＶ－Ｎ１４（Ａｔｔｅｎｕａｔｅｄ ＴＭＶ ｖａｃｃｉｎｅ ｓｔｒａｉｎ）基因组ｃＤＮＡｓ的核苷酸全序列进行了测定，并分析和比较了其基因组的结构和特征。结果表明：普通株基因组（Ｇｅｎｂａｎｋ接收号：ＡＦ１６５１９０）为６３９５个核苷酸：４个功能性开放阅读框

Complete Nucleotide Sequences and Genome Structure of Two Chinese Tobacco Mosaic Virus Isolates Deduced from Full-length Infectious cDNA Clones

The complete nucleotides of two Chinese tobacco mosaic virus (TMV) isolates,TMV-Cv (vulgare strain) and TMV-N14 (an attenuated virus originated from a tomato strain), were determined from their respective full-length infectious cDNA clones and compared with published TMV sequences. The genome structure of TMV-Cv contained 6395 nucleotides,in which four functional open reading frames (ORF), coding for replicase (126kD/183kD), movement protein (MP,30kD) and coat protein (CP, 17.6kD) respectively,could be recognized. TMV-N14 contained 6384 nucleotides in its genome. In contrast to TMV-Cv,five functional ORFs encoding the replicase 98.5kD/126kD/183kD, MP(27kD) and CP(17.6kD) ,respectively, were detected in the TMV-N14 genome. TMV-Cv is 99% homologous to a Korean TMV isolate belonging to the vulgate strain at the nucleotide level. TMV-N14 is 99% homologous to a highly virulent Japanese isolate TMV-L (tomato strain) at the nucleotide level. In TMV-N14,one opal nulation (UGA) occurred in the replicase gene and one ochre nutation (UAA) in the MP gene. The former mutation created a potential,additional ORF within the replicase gene,tbe latter reduced the size of the MP to 27kD. In addition, there were also 13 amino acid substitutions in the replicase gene of TMV-N14 when compared to that of TMV-L. Collectively, these changes may have significant implications in the attenuation of the virulence of TMV-N14.