链霉菌无痕敲除方法研究进展

链霉菌是革兰氏阳性放线菌,能产生大量具有重要价值的天然代谢产物.随着基因测序技术、分子生物学和合成生物学的不断发展,人类对链霉菌家族有了更深的了解,从分子水平对其基因组进行改造的手段也越来越多.通过对链霉菌基因组合理、高效的精简,将提高链霉菌代谢产物的产量和质量,降低底物原料的消耗量.无痕敲除就是开展此项研究工作的重要手段.文章综述了近年来在链霉菌中广泛使用的分子操作方法并重点对链霉菌无痕敲除方法进行了总结.

Progress in markerless knockout of chromosome genes in Streptomycetes

Streptomycetes are Gram-positive bacteria of Actinomycetales. These organisms can produce many economically important secondary metabolites. With the development of molecular biology, gene sequencing technology and synthetic biology, people gained a better understanding of the Streptomyces family. The means to transform genome on the molecular level is also increasing. By simplifying the Streptomyces genome rationally and efficiently, it will improve the yield and quality of the metabolites as well as reduce the consumption of the substrates. Markerless knockout is an important way to carry out genetic modification. Here we describe novel genome modification techniques developed for Streptomyces in recent years with focus on the markerless knockout technologies.