| 人肝脏特异性miR-122表达载体的构建及鉴定 |
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| 引用本文： | 黄增红,刘长梅.人肝脏特异性miR-122表达载体的构建及鉴定[J].生物工程学报,2009,25(4):587-590. |
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| 作者姓名： | 黄增红 刘长梅 |
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| 作者单位： | 1. 中国农业大学生物学院,北京,100193
2. 中国科学院微生物研究所分子病毒室,北京,100101 |
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| 摘 要： | 人肝脏特异性miRNA-122是肝脏中表达丰度最高的miRNA。为研究该miR-122的生物学功能,从HepG2细胞基因组中用PCR的方法扩增了miR-122的前体,构建了miR-122的表达载体pLMP-miR-122。pLMP-miR-122质粒转染人正常肝细胞系L-O2和肝癌细胞系HepG2后,细胞内成熟miRNA-122的表达量显著增加。该质粒与HBV1.3共转染HepG2细胞72h后,HBV的HBs和HBe蛋白水平的表达量均下降,说明miRNA-122参与了HBV基因的复制和表达的调控,为进一步研究miRNA-122的功能和其他一些肝病如HCC的调控机制打下基础。
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| 关 键 词： | miRNA miR-122 pLMP质粒 HBV |
| 收稿时间： | 2008/11/27 0:00:00 |
Construction and identification of the human liver-specific miR-122 expression vector |
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| Zenghong Huang and Changmei Liu.Construction and identification of the human liver-specific miR-122 expression vector[J].Chinese Journal of Biotechnology,2009,25(4):587-590. |
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| Authors: | Zenghong Huang and Changmei Liu |
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| Institution: | College of Biological Science, China Agricultural University, Beijing 100193, China;Laboratory of Molecular Virus, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China |
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| Abstract: | miR-122 is the most abundant miRNA in adult human liver. To study the functions of miR-122 in liver disease,we amplified the precursor of human miR-122 gene by polymerase chain reaction (PCR) from the HepG2 genomic DNA,and then constructed miR-122 expression vector pLMP-miR-122. pLMP-miR-122 could overexpress mature miR-122 when human normal liver cells L-O2 and the hepatoma cells HepG2 were transfected with it. When HepG2 cells were co-transfected with HBV1.3 and pLMP-miR-122,we found that miR-122 could do... |
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| Keywords: | miRNA miR-122 pLMP vector HBV |
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