| 表达乙肝病毒融合表面抗原基因SA-28的二倍体酵母工程菌的选育 |
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| 引用本文： | 陈孝康,袁汉英,潘辉,邬向东.表达乙肝病毒融合表面抗原基因SA-28的二倍体酵母工程菌的选育[J].生物工程学报,2000,16(1):10-12. |
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| 作者姓名： | 陈孝康 袁汉英 潘辉 邬向东 |
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| 作者单位： | [1]复旦大学遗传学研究所遗传工程国家重点实验实验,上海 [2]江西农业大学动物科学技术学院,南 |
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| 基金项目： | 国家高技术研究发展计划项目(863-102-07-01-021) |
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| 摘 要： | 将乙肝病毒融合表达抗原基因ＳＡ－２８的单倍体酵母工程菌Ｙ１９／ＹＦＤ１５８和与其不同接合型的单倍体酵母菌Ｙ９５接合，筛以二倍体酵母工程菌Ｙ９５ｘＹ１９／ＹＦＤ１５８。对两种工程菌的研究表明：三倍体工程菌发酵密度为单倍休工程菌的３倍；表达质粒在二倍体酵母中的稳定性明显高于单倍体工程菌；二倍体工程菌对融合抗原的表达量为单倍体的３倍以上，表达质粒在二倍体细菌中的平均拷贝数略低于单倍体工程菌。
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| 关 键 词： | 酵母 二倍体 乙肝病毒表面抗原 基因表达 选育 |
| 文章编号： | 1000-3061(2000)01-0010-03 |
| 修稿时间： | 1998-12-14 |
The Diploid of Genetically
Engineered Yeast for Expression of Hybrid HBsAg Gene SA-28 |
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| CHEN Xiao-kang ,YUAN Han-ying ,PAN
Hui
WU Xiang-dong
LI Yu-yang.The Diploid of Genetically
Engineered Yeast for Expression of Hybrid HBsAg Gene SA-28[J].Chinese Journal of Biotechnology,2000,16(1):10-12. |
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| Authors: | CHEN Xiao-kang YUAN Han-ying PAN
Hui
WU Xiang-dong
LI Yu-yang |
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| Institution: | CHEN Xiao-kang ,YUAN Han-ying ,PAN
Hui ;
(Institute of Genetitcs and State Key Laboratory of Genetic Engineering,Fudan University,
Shanghai 200433);
WU Xiang-dong ;
(Jiangxi Agricultural University, Nanchang 330045);
LI Yu-yang ;
(Institute of Genetitcs and State Key Laboratory of Genetic Engineering,Fudan University,
Shanghai 200433) |
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| Abstract: | By mating a yeast haploid expressing hybrid HBsAg gene SA 28,Y19/YFD158,with another hapolid Y95,the diploid Y95xY19/YFD158 was constructed.The experiments showed that the cell density of the diploid in fermentation was three time higher than that of the haploid,the stability of expression vector in the diploid was much higher than in the haploid,the xpression level of hybrid HBsAg gene of the diploid was more than three time higher than that of the haploid and the average cope number in diploid cell was a little lower than that in the haploid. |
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| Keywords: | Yeast diploid HBsAg gene expression |
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