| 发酵法生产S-腺苷蛋氨酸前体蛋氨酸补加策略 |
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| 引用本文： | 王杰鹏,谭天伟.发酵法生产S-腺苷蛋氨酸前体蛋氨酸补加策略[J].生物工程学报,2008,24(10):1824-1827. |
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| 作者姓名： | 王杰鹏 谭天伟 |
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| 作者单位： | 北京化工大学生命科学与技术学院,北京,100029 |
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| 基金项目： | 国家自然科学基金，国家高技术研究发展计划(863计划)，国家重点基础研究发展计划(973计划)，北京市自然科学基金，北京市科技计划 |
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| 摘 要： | 利用酿酒酵母菌株高密度发酵法生产S-腺苷蛋氨酸关键的影响因素之一是前体L-蛋氨酸的补加策略.本研究采用一支经过常规诱变处理的S-腺苷蛋氨酸优势积累菌株酿酒酵母SAM0801,通过5 L发酵罐高密度发酵实验研究,考察了6种补加策略,最终确定了L-蛋氨酸的加入时机为30h左右,当茵体干重达到100g/L时,补加量为每罐40gL-蛋氨酸,发酵58 h左右达到最高生物量干重168 g/L,产量14.48 g/L.
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| 关 键 词： | S-腺苷蛋氨酸 酿酒酵母 高密度发酵 L-蛋氨酸 |
| 收稿时间： | 2008/3/11 0:00:00 |
Pre-L-methionine Feeding Strategy for S-adenosyl- L-methionine Fermentative Production |
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| Jiepeng Wang and Tianwei Tan.Pre-L-methionine Feeding Strategy for S-adenosyl- L-methionine Fermentative Production[J].Chinese Journal of Biotechnology,2008,24(10):1824-1827. |
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| Authors: | Jiepeng Wang and Tianwei Tan |
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| Institution: | College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China;College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China |
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| Abstract: | The yield of S-adenosyl-L-methionine (SAM) on high-cell-density fermentation by saccharomyces cerevisiae is mostly affected by the feeding strategy of pre-L-methionine. The mutant strain SAM0801 that could accumulate more SAM was used in this study. Six high-cell-density fermentation experiments in 5 L fermentor were investigated to get the optimal feeding time and amount of L-methionine. The results showed that when 40 g L-methionine was added in the fermentor after 30 h fermentation, a dry cell weight of 100 g/L was achieved. Under this condition, after 58 h fermentation, both the dry cell weight and the yield of SAM reached the maximum, 168 g/L and 14.48 g/L respectively. |
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| Keywords: | S-adenosyl-L-methionine saccharomyces cerevisiae high-cell-density fermentation L-methionine |
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