| 基于甘油的1,3-丙二醇生物合成的代谢局限及其改造策略 |
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| 引用本文： | 杨苗苗,员君华,张欢欢,张国艳,Hossain Zabe,齐向辉.基于甘油的1,3-丙二醇生物合成的代谢局限及其改造策略[J].生物工程学报,2018,34(7):1069-1080. |
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| 作者姓名： | 杨苗苗 员君华 张欢欢 张国艳 Hossain Zabe 齐向辉 |
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| 作者单位： | 江苏大学食品与生物工程学院 |
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| 基金项目： | 国家自然科学基金 (No. 31571806)，国家重点研发计划 (No. 2017YFC1600806)，中国博士后基金 (No. 2017M621657)，江苏省“六大人才高峰”项目 (No. SWYY-018) 资助。 |
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| 摘 要： | 粗甘油是生物柴油生产中的主要副产物,一些微生物可将甘油转化为重要化工原料1,3-丙二醇(1,3-PD),而利用这些微生物野生菌株生物合成1,3-PD会存在一些局限性,如底物抑制、产物抑制等。文中从1,3-丙二醇的甘油生物转化途径与这些局限性出发,总结了生物合成中存在的问题,并针对这些问题提出了一些基于基因敲除或基因过表达等基因工程技术的改造方法,综述了利用基因工程菌生物转化甘油生成1,3-丙二醇的最新研究进展。
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| 关 键 词： | 甘油，1 3-丙二醇，生物转化，生物合成，基因工程 |
| 收稿时间： | 2017/12/25 0:00:00 |
Bottlenecks and modification strategies of 1,3-propanediol biosynthesis from glycerol |
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| Miaomiao Yang,Junhua Yun,Huanhuan Zhang,Guoyan Zhang,Hossain Zabed and Xianghui Qi.Bottlenecks and modification strategies of 1,3-propanediol biosynthesis from glycerol[J].Chinese Journal of Biotechnology,2018,34(7):1069-1080. |
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| Authors: | Miaomiao Yang Junhua Yun Huanhuan Zhang Guoyan Zhang Hossain Zabed and Xianghui Qi |
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| Institution: | School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China,School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China,School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China,School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China,School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China and School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China |
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| Abstract: | Crude glycerol is the main by-product of biodiesel production. A few microorganisms can transfer crude glycerol to 1,3-propanediol (1,3-PD) that is an important chemical material. There exist many limitations such as substrate inhibition, product inhibition when wild strains are used in 1,3-PD biosynthesis. In this review, based on the microbial transformation of 1,3-propanediol from glycerol and its limitations, some strategies using genetic engineering such as knockout or gene overexpression were summarized. The latest research progresses in biosynthesis of 1,3-propanediol from glycerol by genetically engineered strains are discussed. |
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| Keywords: | glycerol 1 3-propanediol bioconversion biosynthesis genetic engineering |
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