利用微卫星标记鉴定杂交水稻冈优22种子纯度的研究

水稻是重要的粮食作物之一,早在70年代我国就已实行了杂交稻的三系配套,目前杂交水稻在我国已大面积种植。由于杂交稻种子的真伪无法从种子形态上鉴别,这给造假者以可乘之机。在正常的情况下,种子的纯度也会因质量控制不严而给农业生产造成大量损失。这些都迫切需要有一种…

Application of Microsatellite Markers for the Seed Purity Examination of a Hybrid Rice, Gangyou-22

For seed commercialization of hybrid rice it is necessary to examine the purity of its seeds before field production,because the seed purity is closely related to their heterosis performance and yield increase.In this research,160 microsatellite markers were used for PCR amplification of rice seedling DNAs of Gangyou-22,which is a major hybrid rice in China,and its parents,Gang46A(CMS line)and CDR22(restorer line).A microsatellite marker,RM168,was screened out for its ability to produce polymorphic bands specific to each of the two parents but different from other 22 restorer lines and 9 cultivars.This provides an accurate and efficient method to examine the purity of a hybrid rice at an earlier time.Amplification of DNAs extracted from seeds and application of two microsatellite markers in one PCR system can further simplify the procedure and improve the accuracy of the seed purity examination.