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| 重组PTD-maxadilan的制备与鉴定 | |
| 引用本文： | 曾乐,余榕捷,徐明芳,陈建苏,王静静,李娟.重组PTD-maxadilan的制备与鉴定[J].生物工程学报,2009,25(11):1739-1745. |
| 作者姓名： | 曾乐 余榕捷 徐明芳 陈建苏 王静静 李娟 |
| 作者单位： | 1. 暨南大学生物工程研究所,广州,510632 2. 暨南大学生命科学技术学院,广州,510632 3. 暨南大学医学院眼科,广州,510632 |
| 基金项目： | 广东省自然科学基金项目(No.06300579);;教育部科学技术研究重点项目(No.207141);;暨南大学青年基金(No.51208018)资助~~ |
| 摘 要： | 为了构建能够有效进入血脑屏障的新型融合蛋白PTD-maxadilan(PTD-MAX),设计编码融合蛋白PTD-MAX基因,克隆到表达载体pKYB,构建重组表达载体pKYB-PTD-MAX,转化大肠杆菌ER2566中。采用IPTG诱导由PTD-MAX、内含肽和几丁质组成的融合蛋白的表达。利用IMPACT(Intein Mediated Purification with an Affinity Chitin-binding Tag)介导的纯化系统制备目的融合蛋白PTD-MAX。所得的目的蛋白经激光飞行质谱测定分子量,结果与理论值相符。动物实验结果表明融合蛋白PTD-MAX能够有效穿越血脑屏障,重组PTD-MAX具有比天然maxadilan更显著(P<0.05)的抑制小鼠摄食的作用。融合蛋白PTD-MAX的构建和制备为其生物学功能的深入开发奠定了基础。 |
| 关 键 词： | PTD-MAX maxadilan 内含肽 血脑屏障 |
| 收稿时间： | 2009/7/18 0:00:00 |
Preparation and identification of recombinant PTD-maxadilan |
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| Le Zeng,Rongjie Yu,Mingfang Xu,Jiansu Chen,Jingjing Wang and Juan Li.Preparation and identification of recombinant PTD-maxadilan[J].Chinese Journal of Biotechnology,2009,25(11):1739-1745. | |
| Authors: | Le Zeng Rongjie Yu Mingfang Xu Jiansu Chen Jingjing Wang and Juan Li |
| Institution: | Bioengineering Institute of Jinan University, Guangzhou 510632, China,Bioengineering Institute of Jinan University, Guangzhou 510632, China,Life Science Department, Jinan University, Guangzhou 510632, China,Ophthalmology Department, Medical College, Jinan University, Guangzhou 510632, China,Bioengineering Institute of Jinan University, Guangzhou 510632, China and Bioengineering Institute of Jinan University, Guangzhou 510632, China |
| Abstract: | In order to construct a novel fusion protein PTD-maxadilan(PTD-MAX) that can enter the blood-brain barrier(BBB) efficiently,a new gene encoding PTD-MAX was synthesized and cloned into the expression vector pKYB.The recombinant vector pKYB-PTD-MAX was transformed into Escherichia coli ER2566.The expression of fusion protein consisting of PTD-MAX,intein and chitin binding domain was induced by IPTG and the target PTD-MAX protein was purified using Intein Mediated Purification with an Affinity Chitin-binding T... |
| Keywords: | PTD-MAX maxadilan intein blood-brain barrier |
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