| 在链霉菌中表达透明颤菌血红蛋白需要异源启动子 |
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| 引用本文： | 崔风文,杨胜利.在链霉菌中表达透明颤菌血红蛋白需要异源启动子[J].生物工程学报,1998,14(1):91-95. |
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| 作者姓名： | 崔风文 杨胜利 |
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| 作者单位： | 中国科学院上海生物工程研究中心 上海 200233 |
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| 摘 要： | 构建了质粒pIJ4083Mpro、pIJ4083\|pro\,pWLD8和pFW3。在浅青紫链霉菌TK24中，启动子探针质粒pIJ4083上的邻苯二酚双加氧酶基因(xylE)不能被透明颤菌血红蛋白基因(vgb)的启动子带动转录，表明vgb启动子在链霉菌中无作用。TK24中，pWLD8和pFW3均能表达透明颤菌血红蛋白(VHb)，pWLD8上可能是由Plac带动vgb的表达；pFW3上vgb基因去掉了非必要部分，克隆在PCR扩增得到的glnA启动子下游，两者连成嵌合基因。
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| 关 键 词： | 浅青紫链霉菌，透明颤菌血红蛋白，异源表达，供氧 |
Heterologous Promoter is Necessary for the Expression of Vitreoscilla Hemoglobin in Streptomyces |
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| Cui Fengwen\ Yang Shengli.Heterologous Promoter is Necessary for the Expression of Vitreoscilla Hemoglobin in Streptomyces[J].Chinese Journal of Biotechnology,1998,14(1):91-95. |
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| Authors: | Cui Fengwen\ Yang Shengli |
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| Abstract: | Plasmids pIJ4083Mpro,pIJ4083 pro,pWLD8 and pFW3 were constructed to study Vitreoscilla hemoglobin(VHb) expression in Streptomyces .The xylE gene on pIJ4083 could not be transcribed by Vitreoscilla hemoglobin gene(vgb) promoter in Streptomyces lividans TK24,it indicated that vgb promoter was functionally inactive in Streptomyces. pWLD8 and pFW3 expressed VHb in TK24.Probably Plac is responsible for VHb expression on pWLD8,while on FW3 vgb gene deleted of its unnecessary parts were cloned down stream from glutamine synthetase gene(glnA)promoter amplified through PCR to form a chimerical gene with PglnA. |
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| Keywords: | Vitreoscilla hemoglobin Streptomyces lividans heterologous expression oxygen supply |
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